国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2014年
11期
858-861,865
,共5页
乳腺肿瘤%缺氧诱导因子1,α亚基%血管内皮生长因子类%血小板衍生生长因子
乳腺腫瘤%缺氧誘導因子1,α亞基%血管內皮生長因子類%血小闆衍生生長因子
유선종류%결양유도인자1,α아기%혈관내피생장인자류%혈소판연생생장인자
Breast neoplasms%Hypoxia-inducible factor 1,alpha subunit%Vascular endothelial growth factors%Platelet derived growth factor
目的 探讨缺氧诱导因子-1α(HIF-1α)、血小板衍生生长因子(PDGF)、促血管内皮生长因子(VEGF)-血管内皮生长因子受体(VEGFR)信号通路效应与乳腺癌发病、转移的相关性.方法 对61例乳腺癌病理标本及17例乳腺增生、纤维瘤标本进行组织形态学和HIF-1α、PDGF-A、VEGF-A、VEGFR-2免疫组织化学研究并进行比较.结果 61例乳腺癌标本中,39例VEGF表达阳性,其中20例(51.3％)阳性表达区域超过肿瘤组织的10％;42例VEGFR表达阳性,有22例(52.4％)阳性表达区域超过肿瘤组织的10％.在VEGF阳性标本中同时VEGFR-2表达阳性的有12例.HIF-1 α表达阳性50例,PDGF表达阳性39例,分别有36例(72.0％)和23例(59.0％)阳性表达区域超过肿瘤组织的10％.VEGFR的表达与患者年龄(x2=11.080,P=0.001)及淋巴结转移(x2=4.699,P =0.046)密切相关,而VEGF(x2 =0.880,P=0.415)、HIF-1α(x2=1.620,P=0.303)及PDGF(x2=0.150,P=0.786)与患者年龄未见明显相关;VEGF(x2=0.677,P=0.437)、HIF-1α(x2=1.394,P =0.323)及PDGF(x2=1.841,P=0.192)与患者淋巴结转移亦未见明显相关.乳腺增生及增生性纤维瘤患者部分标本中亦有HIF-1 α、PDGF-A、VEGF-A和VEGFR-2阳性表达,但均明显低于乳腺癌组织中的表达.结论 HIF-1α、PDGF、VEGF-A、VEGFR-2在乳腺癌组织中均高表达,而VEGFR-2与患者发病年龄及淋巴结转移关系密切,提示上述蛋白可作为诊断、预后和靶向治疗的辅助标志物.
目的 探討缺氧誘導因子-1α(HIF-1α)、血小闆衍生生長因子(PDGF)、促血管內皮生長因子(VEGF)-血管內皮生長因子受體(VEGFR)信號通路效應與乳腺癌髮病、轉移的相關性.方法 對61例乳腺癌病理標本及17例乳腺增生、纖維瘤標本進行組織形態學和HIF-1α、PDGF-A、VEGF-A、VEGFR-2免疫組織化學研究併進行比較.結果 61例乳腺癌標本中,39例VEGF錶達暘性,其中20例(51.3％)暘性錶達區域超過腫瘤組織的10％;42例VEGFR錶達暘性,有22例(52.4％)暘性錶達區域超過腫瘤組織的10％.在VEGF暘性標本中同時VEGFR-2錶達暘性的有12例.HIF-1 α錶達暘性50例,PDGF錶達暘性39例,分彆有36例(72.0％)和23例(59.0％)暘性錶達區域超過腫瘤組織的10％.VEGFR的錶達與患者年齡(x2=11.080,P=0.001)及淋巴結轉移(x2=4.699,P =0.046)密切相關,而VEGF(x2 =0.880,P=0.415)、HIF-1α(x2=1.620,P=0.303)及PDGF(x2=0.150,P=0.786)與患者年齡未見明顯相關;VEGF(x2=0.677,P=0.437)、HIF-1α(x2=1.394,P =0.323)及PDGF(x2=1.841,P=0.192)與患者淋巴結轉移亦未見明顯相關.乳腺增生及增生性纖維瘤患者部分標本中亦有HIF-1 α、PDGF-A、VEGF-A和VEGFR-2暘性錶達,但均明顯低于乳腺癌組織中的錶達.結論 HIF-1α、PDGF、VEGF-A、VEGFR-2在乳腺癌組織中均高錶達,而VEGFR-2與患者髮病年齡及淋巴結轉移關繫密切,提示上述蛋白可作為診斷、預後和靶嚮治療的輔助標誌物.
목적 탐토결양유도인자-1α(HIF-1α)、혈소판연생생장인자(PDGF)、촉혈관내피생장인자(VEGF)-혈관내피생장인자수체(VEGFR)신호통로효응여유선암발병、전이적상관성.방법 대61례유선암병리표본급17례유선증생、섬유류표본진행조직형태학화HIF-1α、PDGF-A、VEGF-A、VEGFR-2면역조직화학연구병진행비교.결과 61례유선암표본중,39례VEGF표체양성,기중20례(51.3％)양성표체구역초과종류조직적10％;42례VEGFR표체양성,유22례(52.4％)양성표체구역초과종류조직적10％.재VEGF양성표본중동시VEGFR-2표체양성적유12례.HIF-1 α표체양성50례,PDGF표체양성39례,분별유36례(72.0％)화23례(59.0％)양성표체구역초과종류조직적10％.VEGFR적표체여환자년령(x2=11.080,P=0.001)급림파결전이(x2=4.699,P =0.046)밀절상관,이VEGF(x2 =0.880,P=0.415)、HIF-1α(x2=1.620,P=0.303)급PDGF(x2=0.150,P=0.786)여환자년령미견명현상관;VEGF(x2=0.677,P=0.437)、HIF-1α(x2=1.394,P =0.323)급PDGF(x2=1.841,P=0.192)여환자림파결전이역미견명현상관.유선증생급증생성섬유류환자부분표본중역유HIF-1 α、PDGF-A、VEGF-A화VEGFR-2양성표체,단균명현저우유선암조직중적표체.결론 HIF-1α、PDGF、VEGF-A、VEGFR-2재유선암조직중균고표체,이VEGFR-2여환자발병년령급림파결전이관계밀절,제시상술단백가작위진단、예후화파향치료적보조표지물.
Objective To discuss the relationship among hypoxia-inducible factor-1α (HIF-1ct),platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF)/VEGFR signaling pathway in breast cancer occurring and metastasis in the early stage.Methods The clinical and pathological features and the expression of HIF-1 α,PDGF-A,VEGF-A and VEGFR-2 were assayed by histomorphology and immunohistochemical staining on tissue specimens from 61 patients of breast cancer and healthy breast tissues healthy breast tissue from 17 patients of benign breast disease.Results VEGF expression was observed in 39 out of 61 malignant breast tissues,more than distribution of positive staining 20 patients (51.3％) of which.VEGFR expression was found in 42 out of 61 lesions,.more than 10％ distribution of positive staining 22 patients (52.4％) of which.Simultaneous expression of VEGF and VEGFR-2 was found in 12 cases.HIF-1α was expressed in 50 and PDGF in 39 out of 61 breast cancer patients,and in 36(72.0％) HIF-1α positive specimens and 23 (59.0％) PDGF positive specimens the staining area was more than 10％.The expression of VEGFR was correlated with patient's age (x2 =11.080,P =0.001) and lymph node metastasis (x2 =4.699,P =0.046).VEGF,HIF-1α and PDGF were not correlated with patient's age (x2 =0.880,P =0.415; x2 =1.620,P =0.303 ; x2 =0.150,P =0.786) and lymph node metastasis (x2 =0.677,P =0.437 ; x2 =1.394,P=0.323;x2 =1.841,P=0.192).Conclusion HIF-1α,PDGF,VEGF and VEGFR-2 express in breast tumor tissues at a high level,and VEGFR-2 is correlated with patient's age and lymph node metastasis.Abovementioned proteins can be used as markers for diagnosis,prognosis and targeted therapy.
