国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2015年
1期
5-9
,共5页
陈明霞%张蔚%曲建力%李强%王海
陳明霞%張蔚%麯建力%李彊%王海
진명하%장위%곡건력%리강%왕해
乳腺肿瘤%肿瘤转移%斯伐他汀%甲状旁腺素
乳腺腫瘤%腫瘤轉移%斯伐他汀%甲狀徬腺素
유선종류%종류전이%사벌타정%갑상방선소
Breast neoplasms%Neoplasm metastasis%Simvastatin%Parathyroid hormone
目的 观察辛伐他汀在裸鼠模型中对乳腺癌骨转移的作用.方法采用完全随机分组方法 将60只裸鼠分为3组,每组20只,裸鼠左心腔注射乳腺癌骨转移细胞株(MDA-MB-231),7d后,分别皮下注射辛伐他汀、生理盐水及无任何处理(2次/周,19 d).应用图像分析软件评估骨转移瘤的面积.随后处死裸鼠,用放射免疫法检测骨转移癌髓腔内甲状旁腺素相关蛋白(PTHrP)浓度;应用骨密度检测软件进行组织形态学分析,计数骨转移灶每毫米癌组织与临近骨小梁之间破骨细胞的数量.计量资料比较采用方差分析,P＜0.05为差异有统计学意义.结果 与生理盐水组和无处理组相比,注射辛伐他汀的裸鼠骨转移癌面积明显减小(0.51±0.18 mm2 vs 2.13±1.24 mm2 vs 2.29±1.22 mm2;F=15.600,P=0.002; F=15.673,P=0.001),骨转移癌周围髓腔内PTHrP浓度明显降低(0.98±0.20 pmol/L vs 2.11±0.31 pmol/L vs 1.99±0.29 pmol/L;F=61.469,P＜0.001;F=58.274,P＜0.001),并且其转移灶破骨细胞的数量明显减少(4.00±1.73个/mm vs 11.40 ±4.93个/mm vs 10.91±3.87个/mm;F=17.820,P=0.001,F=17.184,P=0.002).结论 辛伐他汀能够降低乳腺癌细胞PTHrP的分泌,从而抑制乳腺癌细胞在骨内生长及其对骨质的破坏.
目的 觀察辛伐他汀在裸鼠模型中對乳腺癌骨轉移的作用.方法採用完全隨機分組方法 將60隻裸鼠分為3組,每組20隻,裸鼠左心腔註射乳腺癌骨轉移細胞株(MDA-MB-231),7d後,分彆皮下註射辛伐他汀、生理鹽水及無任何處理(2次/週,19 d).應用圖像分析軟件評估骨轉移瘤的麵積.隨後處死裸鼠,用放射免疫法檢測骨轉移癌髓腔內甲狀徬腺素相關蛋白(PTHrP)濃度;應用骨密度檢測軟件進行組織形態學分析,計數骨轉移竈每毫米癌組織與臨近骨小樑之間破骨細胞的數量.計量資料比較採用方差分析,P＜0.05為差異有統計學意義.結果 與生理鹽水組和無處理組相比,註射辛伐他汀的裸鼠骨轉移癌麵積明顯減小(0.51±0.18 mm2 vs 2.13±1.24 mm2 vs 2.29±1.22 mm2;F=15.600,P=0.002; F=15.673,P=0.001),骨轉移癌週圍髓腔內PTHrP濃度明顯降低(0.98±0.20 pmol/L vs 2.11±0.31 pmol/L vs 1.99±0.29 pmol/L;F=61.469,P＜0.001;F=58.274,P＜0.001),併且其轉移竈破骨細胞的數量明顯減少(4.00±1.73箇/mm vs 11.40 ±4.93箇/mm vs 10.91±3.87箇/mm;F=17.820,P=0.001,F=17.184,P=0.002).結論 辛伐他汀能夠降低乳腺癌細胞PTHrP的分泌,從而抑製乳腺癌細胞在骨內生長及其對骨質的破壞.
목적 관찰신벌타정재라서모형중대유선암골전이적작용.방법채용완전수궤분조방법 장60지라서분위3조,매조20지,라서좌심강주사유선암골전이세포주(MDA-MB-231),7d후,분별피하주사신벌타정、생리염수급무임하처리(2차/주,19 d).응용도상분석연건평고골전이류적면적.수후처사라서,용방사면역법검측골전이암수강내갑상방선소상관단백(PTHrP)농도;응용골밀도검측연건진행조직형태학분석,계수골전이조매호미암조직여림근골소량지간파골세포적수량.계량자료비교채용방차분석,P＜0.05위차이유통계학의의.결과 여생리염수조화무처리조상비,주사신벌타정적라서골전이암면적명현감소(0.51±0.18 mm2 vs 2.13±1.24 mm2 vs 2.29±1.22 mm2;F=15.600,P=0.002; F=15.673,P=0.001),골전이암주위수강내PTHrP농도명현강저(0.98±0.20 pmol/L vs 2.11±0.31 pmol/L vs 1.99±0.29 pmol/L;F=61.469,P＜0.001;F=58.274,P＜0.001),병차기전이조파골세포적수량명현감소(4.00±1.73개/mm vs 11.40 ±4.93개/mm vs 10.91±3.87개/mm;F=17.820,P=0.001,F=17.184,P=0.002).결론 신벌타정능구강저유선암세포PTHrP적분비,종이억제유선암세포재골내생장급기대골질적파배.
Objective To observe the effect of simvastatin on bone metastasis of breast cancer in nude mice model.Methods Sixty mice were divided into three groups randomly with 20 in each group.Mice were inoculated with MDA-MB-231 cells into the left cardiac ventricle.After 7 days,mice were treated with either simvastatin,saline,or nothing twice per week for 19 days.The area of osteolytic metastases was subsequently measured in long bones of all mice using an image analysis system.After sacrifice,parathyroid hormone-related protein (PTHrP) concentrations in bone marrow from all mice were determined using a two-site immunoradiometric assay.Osteoclast number expressed per millimeter of tumor/bone interface was assessed using an OsteoMeasure System.Measured data were compared with analysis of variance,and P ＜ 0.05 for the difference was statistically significant.Results The area of osteolytic lesions was significantly lower in mice treated with simvastatin compared with mice receiving saline and no treatment (0.51 ±0.18 mm2 vs 2.13 ± 1.24 mm2 vs 2.29 ± 1.22 mm2 ; F =15.600,P =0.002 ; F =15.673,P =0.001).In addition,treatment with simvastatin decreased the concentrations of PTHrP in bone marrow plasma (0.98 ±0.20 pmol/L vs 2.11 ±0.31 pmoL/L vs 1.99 ± 0.29 pmol/L; F =61.469,P ＜ 0.001 ; F =58.274,P ＜ 0.001) and the osteoclast numbers per millimeter of tumor/bone interface (4.00 ± 1.73 vs 11.40 ±4.93 vs 10.91 ± 3.87 ; F =17.820,P =0.001 ; F =17.184,P =0.002) compared with controls.Conclusion Simvastatin may reduce the production of PTHrP of breast cancer cells,which suppresses the development of destructive bone lesions as well as the growth of breast cancer cells in bone.
