国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2014年
12期
834-836
,共3页
小鼠%腹部%心脏移植%模型
小鼠%腹部%心髒移植%模型
소서%복부%심장이식%모형
Mice%Abdomen%Heart transplantation%Models
目的 建立小鼠异位心脏移植模型,为进行移植免疫研究提供动物模型.方法 昆明(KM)小鼠50只,随机分供、受体两组,借鉴大鼠异位心脏移植Ono式模型经验,并加以改良,将供体升主动脉、肺动脉分别与受体的腹主动脉和下腔静脉行端侧连续吻合.结果 成功建立了小鼠腹腔异位心脏移植模型,实验成功率为84％ (21/25).供、受体的手术时间分别为(11.0±l.0)min和(60.0 ±2.0)min.结论 熟练掌握显微外科技术后,可以成功建立小鼠异位心脏移植模型.
目的 建立小鼠異位心髒移植模型,為進行移植免疫研究提供動物模型.方法 昆明(KM)小鼠50隻,隨機分供、受體兩組,藉鑒大鼠異位心髒移植Ono式模型經驗,併加以改良,將供體升主動脈、肺動脈分彆與受體的腹主動脈和下腔靜脈行耑側連續吻閤.結果 成功建立瞭小鼠腹腔異位心髒移植模型,實驗成功率為84％ (21/25).供、受體的手術時間分彆為(11.0±l.0)min和(60.0 ±2.0)min.結論 熟練掌握顯微外科技術後,可以成功建立小鼠異位心髒移植模型.
목적 건립소서이위심장이식모형,위진행이식면역연구제공동물모형.방법 곤명(KM)소서50지,수궤분공、수체량조,차감대서이위심장이식Ono식모형경험,병가이개량,장공체승주동맥、폐동맥분별여수체적복주동맥화하강정맥행단측련속문합.결과 성공건립료소서복강이위심장이식모형,실험성공솔위84％ (21/25).공、수체적수술시간분별위(11.0±l.0)min화(60.0 ±2.0)min.결론 숙련장악현미외과기술후,가이성공건립소서이위심장이식모형.
Objective To establish a mouse model of heterotopic heart transplantation and construct an animal model for the study of transplantation immunity.Methods According to the surgical procedures of Ono's pattern in mice cardiac heterotopic transplantation model,50 Kunming mice were divided into donors and recipients randomly.The donor heart aorta and the recipient ventral aorta,the donor pulmonary artery and the recipient inferior caval vein,were anastomosed by using the end-to-side suture technique respectively.Results The mouse model of heterotopic heart transplantation was established successfully with a success rate of 84％ (21/25).The average time of donor operation and recipient operation was (11.0 ± 1.0) min and(60.0 ± 2.0) min respectively.Conclusions In the base of mastering the technique of operation facility,the model of mice heart heterotopic transplantation would be established successfully.
