国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2015年
1期
1-5,13
,共6页
陈超%白瑞璞%何兴%潘卫庆
陳超%白瑞璞%何興%潘衛慶
진초%백서박%하흥%반위경
日本血吸虫%吡喹酮%肝纤维化%微小RNA
日本血吸蟲%吡喹酮%肝纖維化%微小RNA
일본혈흡충%필규동%간섬유화%미소RNA
Schistosoma japonicum%Praziquantel%Liver fibrosis%MicroRNA
目的 研究吡喹酮对日本血吸虫虫卵引起的肝纤维化的治疗作用及其可能的机制. 方法 选用BALB/c小鼠建立日本血吸虫感染小鼠模型,实验组以250 mg/(kg·d)吡喹酮连续用药3d进行杀虫,再以600 mg/(kg·d)连续给药30 d进行抗纤维化治疗;对照组仅进行吡喹酮杀虫.以肝组织羟脯氨酸含量等指标评定小鼠肝纤维化的程度,并通过实时荧光定量PCR (real-time PCR)检测各组肝组织中某些微小RNA (microRNA,miRNA)的表达水平.各组检测结果以t检验进行统计学分析.结果 经过吡喹酮治疗后,小鼠肝脏羟脯氨酸含量等肝纤维化指标明显降低,小鼠肝脏羟脯氨酸含量:感染后第75天,感染组、杀虫组、治疗组依次为(0.86±0.07)、(0.66±0.06)、(0.25±0.05) mg/g肝湿重,治疗组低于感染组和杀虫组(t=12.86,P＜0.01).同时小鼠肝脏的部分miRNA表达水平发生了明显改变,治疗组与杀虫组相比较,Col I、miR-223、miR-146b、miR-142-5p、miR-199a-5p、miR-34c*、miR-195依次下调了62％、38％、75％、77％、40％、54％和56％. 结论 吡喹酮对日本血吸虫病肝纤维化有一定的治疗作用,其作用可能与调节某些宿主miRNA的表达水平有关.
目的 研究吡喹酮對日本血吸蟲蟲卵引起的肝纖維化的治療作用及其可能的機製. 方法 選用BALB/c小鼠建立日本血吸蟲感染小鼠模型,實驗組以250 mg/(kg·d)吡喹酮連續用藥3d進行殺蟲,再以600 mg/(kg·d)連續給藥30 d進行抗纖維化治療;對照組僅進行吡喹酮殺蟲.以肝組織羥脯氨痠含量等指標評定小鼠肝纖維化的程度,併通過實時熒光定量PCR (real-time PCR)檢測各組肝組織中某些微小RNA (microRNA,miRNA)的錶達水平.各組檢測結果以t檢驗進行統計學分析.結果 經過吡喹酮治療後,小鼠肝髒羥脯氨痠含量等肝纖維化指標明顯降低,小鼠肝髒羥脯氨痠含量:感染後第75天,感染組、殺蟲組、治療組依次為(0.86±0.07)、(0.66±0.06)、(0.25±0.05) mg/g肝濕重,治療組低于感染組和殺蟲組(t=12.86,P＜0.01).同時小鼠肝髒的部分miRNA錶達水平髮生瞭明顯改變,治療組與殺蟲組相比較,Col I、miR-223、miR-146b、miR-142-5p、miR-199a-5p、miR-34c*、miR-195依次下調瞭62％、38％、75％、77％、40％、54％和56％. 結論 吡喹酮對日本血吸蟲病肝纖維化有一定的治療作用,其作用可能與調節某些宿主miRNA的錶達水平有關.
목적 연구필규동대일본혈흡충충란인기적간섬유화적치료작용급기가능적궤제. 방법 선용BALB/c소서건립일본혈흡충감염소서모형,실험조이250 mg/(kg·d)필규동련속용약3d진행살충,재이600 mg/(kg·d)련속급약30 d진행항섬유화치료;대조조부진행필규동살충.이간조직간포안산함량등지표평정소서간섬유화적정도,병통과실시형광정량PCR (real-time PCR)검측각조간조직중모사미소RNA (microRNA,miRNA)적표체수평.각조검측결과이t검험진행통계학분석.결과 경과필규동치료후,소서간장간포안산함량등간섬유화지표명현강저,소서간장간포안산함량:감염후제75천,감염조、살충조、치료조의차위(0.86±0.07)、(0.66±0.06)、(0.25±0.05) mg/g간습중,치료조저우감염조화살충조(t=12.86,P＜0.01).동시소서간장적부분miRNA표체수평발생료명현개변,치료조여살충조상비교,Col I、miR-223、miR-146b、miR-142-5p、miR-199a-5p、miR-34c*、miR-195의차하조료62％、38％、75％、77％、40％、54％화56％. 결론 필규동대일본혈흡충병간섬유화유일정적치료작용,기작용가능여조절모사숙주miRNA적표체수평유관.
Objective To investigate the role and mechanism of praziquantel against Schistosoma japonicum egg induced liver fibrosis.Methods BALB/c mice were infected with Schistosoma japonicum as experimental mouse model.In the experimental group,praziquantel at 250 mg/(kg·d) for consecutive 3 days as insecticide treatment and at 600 mg/(kg·d) for 30 days of continuous administration as antifibrotic therapy while in the control group,the mice only given praziquantel insecticide treatment.The extent of liver fibrosis was evaluated by detection on liver hydroxyproline content and other indicators,and by detection on certain micro RNA (microRNA,miRNA) expression levels in liver tissues with real-time quantitative PC R (real-time PCR).Test results of each group were statistically analyzed with t test.Results liver hydroxyproline content of hepatic fibrosis was significantly reduced due to praziquantel treatment.Liver hydroxyproline content in D75 infection group,insecticide group and treatment group were (0.86±0.07),(0.66±0.06) and (0.25±0.05) mg/g liver wet weight respectively,the hydroxyproline content in the treatment group is lower than that in the infection group and insecticide group(t=12.86,P＜0.01).While some significant changes in miRNA expression levels of mouse liver exhibited in the treatment group compared with that in the insecticide group such as the down-regulation of Col I by 62％,miR-223 by 38％,miR-146b by 75％,miR-142-5p by 77％,miR-199a-5p by 40％,miR-34c* by 54％,and miR-195 by 56％.Conclusion Praziquantel may have certain therapeutic effects on schistosomiasis japonica liver fibrosis by modulating the expression of certain host miRNA expression levels.
