CAJ | 학술논문

目的：观察七十味珍珠丸（RNSP）对大鼠脑缺血再灌注损伤的保护作用及抗凋亡机制。方法采用大脑中动脉阻塞模型，实验分组：假手术组、模型组、溶媒对照组和RNSP组。神经功能缺失评分观察大鼠神经缺失体征，红四氮唑染色测量脑组织梗死面积，及Western blot检测海马组织Bcl-2/Bax比值和caspase-3的蛋白表达。结果与假手术组比较，模型组大鼠神经缺失体征明显，脑梗死面积明显增加，Bcl-2/Bax比值减小，caspase-3蛋白表达明显升高。而与模型组比较，RNSP组大鼠神经缺失体征明显改善，脑梗死面积减少，Bcl-2/Bax比值增大，caspase-3蛋白表达明显降低。结论 RNSP对大鼠脑缺血再灌注损伤具有保护作用，与其上调Bcl-2/Bax比值，减少caspase-3蛋白表达的抗凋亡作用有关。
목적：관찰칠십미진주환（RNSP）대대서뇌결혈재관주손상적보호작용급항조망궤제。방법채용대뇌중동맥조새모형，실험분조：가수술조、모형조、용매대조조화RNSP조。신경공능결실평분관찰대서신경결실체정，홍사담서염색측량뇌조직경사면적，급Western blot검측해마조직Bcl-2/Bax비치화caspase-3적단백표체。결과여가수술조비교，모형조대서신경결실체정명현，뇌경사면적명현증가，Bcl-2/Bax비치감소，caspase-3단백표체명현승고。이여모형조비교，RNSP조대서신경결실체정명현개선，뇌경사면적감소，Bcl-2/Bax비치증대，caspase-3단백표체명현강저。결론 RNSP대대서뇌결혈재관주손상구유보호작용，여기상조Bcl-2/Bax비치，감소caspase-3단백표체적항조망작용유관。
Objective To observe the protective and anti-apoptosis effects of Rannasangpei (RNSP) on brain ischemia and reperfusion injury in rats. Methods Middle cerebral artery occlusion (MCAO) model was established and the groups were divided as sham group, MCAO group, vehicle + MCAO group and RNSP + MCAO group. Neuronal deficient signs, brain infarct area, the ratio of Bcl-2/Bax and the expression of caspase-3 were evaluated by neuronal deficient score, TTC (2,3,5-Triphenyltetrazolium chloride) staining and Western blot respectively. Results Compared with those parameters in sham group, the neuronal deficient signs, infarct area and caspase-3 expression increased evidently while the ratio of Bcl-2/Bax decreased markedly in MCAO group. But in RNSP+MCAO group, the neuronal deficient signs, infarct area and cas?pase-3 expression decreased greatly while the ratio of Bcl-2/Bax increased markedly compared with those parameters in MCAO group. Conclusion RNSP may have protective effects on brain ischemia and reperfusion, which is related to its an?ti-apoptosis role indicated by upregulation of Bcl-2/Bax ratios and downregulation of caspase-3.