天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2015年
2期
117-120
,共4页
马懿江%何庆%胡志东%冯靖
馬懿江%何慶%鬍誌東%馮靖
마의강%하경%호지동%풍정
肺气肿%间歇低氧%氧化应激%超氧化物歧化酶%过氧化氢酶%丙二醛%凝血因子
肺氣腫%間歇低氧%氧化應激%超氧化物歧化酶%過氧化氫酶%丙二醛%凝血因子
폐기종%간헐저양%양화응격%초양화물기화매%과양화경매%병이철%응혈인자
pulmonary emphysema%intermittent hypoxia%oxidative stress%superoxide dismutase%catalase%malondialde-hyde%blood coagulation factors
目的：建立肺气肿合并间歇低氧（IH）的重叠综合征（OS）大鼠模型，探讨OS大鼠肝脏炎性损伤及其凝血功能的变化。方法将60只雄性Wistar大鼠随机分成正常组（A组）、IH组（B组）、肺气肿组（C组）和IH合并肺气肿组（D组）。通过对大鼠进行16周的熏烟暴露造成大鼠肺气肿；从13周开始，同时施加程控预制的间歇低氧/再氧合（IH/ROX）处理对大鼠进行IH暴露4周。暴露结束后取各组大鼠肺和肝脏组织于光镜下观察并计算肝脏炎性损伤病理评分。取肝组织匀浆采用ELISA方法测定其超氧化物歧化酶（SOD）活性、过氧化氢酶（CAT）活性和丙二醛（MDA）浓度。检测血浆纤维蛋白原（FIB），血浆因子FⅧ促凝活性（FⅧ：C）、血管性血友病因子抗原（vWF：Ag）和抗凝血酶活性（AT：A）水平。结果 D组肝脏炎性损伤病理评分和凝血因子FIB、FⅧ：C、vWF：Ag水平均高于A、B、C组，而SOD和CAT活性以及AT：A水平低于其他3组（均P<0.05）。FIB、vWF：Ag、FⅧ：C、AT：A与SOD呈明显相关（r分别为-0.905、-0.941、-0.946和0.817，均P<0.01）。结论 OS动物模型下IH和肺气肿在导致氧化应激和高凝状态上具有一定叠加效应，可引起更强的肝脏炎症反应和血栓前状态。
目的：建立肺氣腫閤併間歇低氧（IH）的重疊綜閤徵（OS）大鼠模型，探討OS大鼠肝髒炎性損傷及其凝血功能的變化。方法將60隻雄性Wistar大鼠隨機分成正常組（A組）、IH組（B組）、肺氣腫組（C組）和IH閤併肺氣腫組（D組）。通過對大鼠進行16週的熏煙暴露造成大鼠肺氣腫；從13週開始，同時施加程控預製的間歇低氧/再氧閤（IH/ROX）處理對大鼠進行IH暴露4週。暴露結束後取各組大鼠肺和肝髒組織于光鏡下觀察併計算肝髒炎性損傷病理評分。取肝組織勻漿採用ELISA方法測定其超氧化物歧化酶（SOD）活性、過氧化氫酶（CAT）活性和丙二醛（MDA）濃度。檢測血漿纖維蛋白原（FIB），血漿因子FⅧ促凝活性（FⅧ：C）、血管性血友病因子抗原（vWF：Ag）和抗凝血酶活性（AT：A）水平。結果 D組肝髒炎性損傷病理評分和凝血因子FIB、FⅧ：C、vWF：Ag水平均高于A、B、C組，而SOD和CAT活性以及AT：A水平低于其他3組（均P<0.05）。FIB、vWF：Ag、FⅧ：C、AT：A與SOD呈明顯相關（r分彆為-0.905、-0.941、-0.946和0.817，均P<0.01）。結論 OS動物模型下IH和肺氣腫在導緻氧化應激和高凝狀態上具有一定疊加效應，可引起更彊的肝髒炎癥反應和血栓前狀態。
목적：건립폐기종합병간헐저양（IH）적중첩종합정（OS）대서모형，탐토OS대서간장염성손상급기응혈공능적변화。방법장60지웅성Wistar대서수궤분성정상조（A조）、IH조（B조）、폐기종조（C조）화IH합병폐기종조（D조）。통과대대서진행16주적훈연폭로조성대서폐기종；종13주개시，동시시가정공예제적간헐저양/재양합（IH/ROX）처리대대서진행IH폭로4주。폭로결속후취각조대서폐화간장조직우광경하관찰병계산간장염성손상병리평분。취간조직균장채용ELISA방법측정기초양화물기화매（SOD）활성、과양화경매（CAT）활성화병이철（MDA）농도。검측혈장섬유단백원（FIB），혈장인자FⅧ촉응활성（FⅧ：C）、혈관성혈우병인자항원（vWF：Ag）화항응혈매활성（AT：A）수평。결과 D조간장염성손상병리평분화응혈인자FIB、FⅧ：C、vWF：Ag수평균고우A、B、C조，이SOD화CAT활성이급AT：A수평저우기타3조（균P<0.05）。FIB、vWF：Ag、FⅧ：C、AT：A여SOD정명현상관（r분별위-0.905、-0.941、-0.946화0.817，균P<0.01）。결론 OS동물모형하IH화폐기종재도치양화응격화고응상태상구유일정첩가효응，가인기경강적간장염증반응화혈전전상태。
Objective To establish the rat overlap syndrome (OS) model of intermittent hypoxia (IH) combined with pulmonary emphysema and to explore its connection with hepatic oxidative stress, inflammatory status in the live and coagu?lation profile. Methods Male Wistar rats (n=60) were randomly divided into four groups:control group (A), IH group (B), pulmonary emphysema group (C) and OS group (D). The rat model of pulmonary emphysema was established by exposing rats in smoke for 16 weeks. From the 13th week, pre-programmed intermittent hypoxia/re-oxygenation (IH/ROX) exposure was given in the meantime of smoke exposure in OS group. Liver tissues were sectioned or triturated for pathological scoring or for detecting expression levels of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) respectively. Se?rum levels of coagulant/anticoagulant factors such as antithrombin (AT), fibrinogen (FIB), von Willebrand factor(vWF) and FactorⅧ(FⅧ) were also evaluated using biochemistry analysis. Results The levels of pathological scores and coagulant factors(FIB, FⅧ:C and vWF:Ag)were significantly higher in group D than those in group A, B and C. The values of SOD, CAT and AT were significantly lower in group D than those in other three groups. Serum levels of FIB, vWF:Ag, FⅧ:C and AT:A correlated with SOD(r equal to-0.905、-0.941、-0.946 and 0.817 respective,P<0.01). Conclusion In rat overlap syndrome when IH combined with pulmonary emphysema, hepatic inflammation and coagulability present mutual promotion effect and produce a more significant liver-derivative inflammatory and prothrombotic status.
