CAJ | 학술논문

目的：探讨间歇低氧（IH）对大鼠肺组织RhoA/ROCK通路及肺血管新生肌化的影响。方法将40只Wistar雄性大鼠随机分为对照组和IH组各20只，对照组：间歇正常氧暴露（21%O2）；IH组：IH暴露(5%~21%O2），持续4周。暴露结束后，2组均采用Real-time PCR和Western blot检测肺组织RhoA、ROCK mRNA和蛋白表达情况，免疫组化法测定肺组织和肺动脉的增殖细胞核抗原（PCNA）及α-平滑肌肌动蛋白（SM-α-actin）的表达情况。结果 IH组肺组织RhoA mRNA（0.463±0.067 vs 0.182±0.040）、ROCK mRNA（0.384±0.062 vs 0.192±0.052）、RhoA蛋白（0.827±0.065 vs 0.424±0.075）及ROCK蛋白（0.488±0.088 vs 0.336±0.102）表达水平均高于对照组（均P<0.05）；IH组肺组织PCNA[（54.67±1.80）%vs (9.14±0.91）%]、肺动脉PCNA[（49.40±1.21）%vs (8.38±1.13）%]、肺组织SM-α-actin [（42.66±1.63）%vs (35.44±1.41）%]与肺动脉SM-α-actin[（62.62±2.53）%vs (45.54±2.58）%]表达水平均明显高于对照组（均P<0.05）。结论 RhoA/ROCK传导通路在IH所致肺动脉高压发病机制中可能起着重要作用；IH可通过促进肺动脉的新生肌化进一步促进肺动脉压力升高。
목적：탐토간헐저양（IH）대대서폐조직RhoA/ROCK통로급폐혈관신생기화적영향。방법장40지Wistar웅성대서수궤분위대조조화IH조각20지，대조조：간헐정상양폭로（21%O2）；IH조：IH폭로(5%~21%O2），지속4주。폭로결속후，2조균채용Real-time PCR화Western blot검측폐조직RhoA、ROCK mRNA화단백표체정황，면역조화법측정폐조직화폐동맥적증식세포핵항원（PCNA）급α-평활기기동단백（SM-α-actin）적표체정황。결과 IH조폐조직RhoA mRNA（0.463±0.067 vs 0.182±0.040）、ROCK mRNA（0.384±0.062 vs 0.192±0.052）、RhoA단백（0.827±0.065 vs 0.424±0.075）급ROCK단백（0.488±0.088 vs 0.336±0.102）표체수평균고우대조조（균P<0.05）；IH조폐조직PCNA[（54.67±1.80）%vs (9.14±0.91）%]、폐동맥PCNA[（49.40±1.21）%vs (8.38±1.13）%]、폐조직SM-α-actin [（42.66±1.63）%vs (35.44±1.41）%]여폐동맥SM-α-actin[（62.62±2.53）%vs (45.54±2.58）%]표체수평균명현고우대조조（균P<0.05）。결론 RhoA/ROCK전도통로재IH소치폐동맥고압발병궤제중가능기착중요작용；IH가통과촉진폐동맥적신생기화진일보촉진폐동맥압력승고。
Objective To explore the effect of intermittent hypoxia (IH) on RhoA/ROCK pathway in lung and on the muscularization in pulmonary vascular in rat model. Methods Wistar rats (n=40) were randomly divided into two groups:the normal oxygen control group (n=20) and the IH group ( n=20). For 4 weeks, rats in control group and IH group were ex?posed to intermittent normal oxygen (21%O2) or IH (5%-21%O2) respectively. Then, mRNA transcription and protein trans?lation levels of RhoA/ROCK were examined by Real-time PCR and Western blot. Expression of proliferation cell nuclear an?tigen (PCNA) andα-smooth muscle actin (SM-α-actin ) of lung and pulmonary artery were detected by immunohistochemis?try. Results RhoA mRNA transcription level(0.463 ± 0.067 vs 0.182 ± 0.040), ROCK mRNA transcription level(0.384 ± 0.062 vs 0.192 ± 0.052), RhoA protein expression level(0.827 ± 0.065 vs 0.424 ± 0.075)and ROCK protein expression level (0.488±0.088 vs 0.336±0.102)were higher in IH group than those in control group(P<0.05);Levels of PCNA in lung tissue [(54.67±1.80)%vs (9.14±0.91)%], PCNA in pulmonary artery [(49.40±1.21)%vs (8.38±1.13)%], SM-α-actin in lung tis?sue [(42.66±1.63)%vs (35.44±1.41)%] and SM-α-actin in pulmonary artery [(62.62±2.53)%vs (45.54±2.58)%] were also higher in IH group than those in control group(P<0.05). Conclusion Rho/ROCK pathway may play an important role in developing pulmonary hypertension (PH) associated with IH;and IH can promote the muscularization in pulmonary vascular to accelerate PH.