CAJ | 학술논문

目的：探讨人乳头状瘤病毒（HPV）E6/E7 mRNA检测对高危型人乳头瘤病毒（HR-HPV）感染宫颈病变筛查的临床价值。方法选择265例HR-HPV感染者，其中100例病理结果为正常宫颈/慢性炎症（对照组），88例宫颈上皮内瘤变（CIN）Ⅰ级，33例CINⅡ级，28例CINⅢ级，16例宫颈癌患者的宫颈脱落细胞标本，采用支链DNA（b-DNA）技术检测HPV E6/E7 mRNA的表达情况。结果 CINⅡ级（81.82%）、CINⅢ级（89.29%）、宫颈癌组（100.00%）的HPV E6/E7 mRNA阳性率高于对照组（20.00%）和CINⅠ级（35.23%），差异有统计学意义，其余各组间比较差异无统计学意义；CINⅡ级、CINⅢ级、宫颈癌组的HPV E6/E7 mRNA表达量也高于对照组和CINⅠ级，差异有统计学意义，其余各组间比较差异无统计学意义；高级别鳞状上皮内瘤变（HSIL）和癌组的HPV E6/E7 mRNA阳性率及表达量显著高于正常、非典型鳞状细胞（ASC）和低级别鳞状上皮内瘤变（LSIL）组（P<0.05）。结论 HPV E6/E7 mRNA的表达可能提示了病毒的活性和病变的进展程度，可能成为筛查出高级别宫颈病变的有效指标，可以作为宫颈癌前病变筛查的辅助方法。
목적：탐토인유두상류병독（HPV）E6/E7 mRNA검측대고위형인유두류병독（HR-HPV）감염궁경병변사사적림상개치。방법선택265례HR-HPV감염자，기중100례병리결과위정상궁경/만성염증（대조조），88례궁경상피내류변（CIN）Ⅰ급，33례CINⅡ급，28례CINⅢ급，16례궁경암환자적궁경탈락세포표본，채용지련DNA（b-DNA）기술검측HPV E6/E7 mRNA적표체정황。결과 CINⅡ급（81.82%）、CINⅢ급（89.29%）、궁경암조（100.00%）적HPV E6/E7 mRNA양성솔고우대조조（20.00%）화CINⅠ급（35.23%），차이유통계학의의，기여각조간비교차이무통계학의의；CINⅡ급、CINⅢ급、궁경암조적HPV E6/E7 mRNA표체량야고우대조조화CINⅠ급，차이유통계학의의，기여각조간비교차이무통계학의의；고급별린상상피내류변（HSIL）화암조적HPV E6/E7 mRNA양성솔급표체량현저고우정상、비전형린상세포（ASC）화저급별린상상피내류변（LSIL）조（P<0.05）。결론 HPV E6/E7 mRNA적표체가능제시료병독적활성화병변적진전정도，가능성위사사출고급별궁경병변적유효지표，가이작위궁경암전병변사사적보조방법。
Objective To explore the clinical value of examining HPV E6/E7 mRNA level in assessing cervical le?sions infected with high-risk human papillomavirus (HR-HPV). Methods The cervical epithelial cells were collected from 265 patients with HR-HPV infection, including 100 cases of neoplasia free/inflammation group (control group), 88 cas?es of cervical intraepithelial neoplasia (CIN)Ⅰ, 33 cases of CINⅡ, 28 cases of CINⅢand 16 cases of cervical carcinoma and the transcription of HPV E6/E7 mRNA level was examined using branched DNA (b-DNA) technology. Results The positive rate HPV E6/E7 mRNA were higher in CIN Ⅱ(81.82%), CINⅢ(89.29%) and cervical cancer group (100.00%) than tthat in control group (20.00%) and CINⅠ(35.23%) with significant difference, and there were no significant differences between other groups;The positive rate and transcription level of HPV E6/E7 mRNA in HSIL (high grade squamous intraepi?thelial lesion)and cancer group were significantly higher than normal, ASC(atypical squamous cell carcinoma) and LSIL(low grade squamous intraepithelial lesion) group (P<0.05). Conclusion The transcription level of HPV E6/E7 mRNA may re?flect the activity of the virus and the progression of disease, and could be use as an effective indicator to screen high grade cervical pathological changes and a complementary method of cervical lesion screening.