西安交通大学学报（医学版）
西安交通大學學報（醫學版）
서안교통대학학보（의학판）
JOURNAL OF XI'AN JIAOTONG UNIVERSITY(MEDICAL SCIENCES)
2015年
2期
219-224
,共6页
常曦%焦谊%陆剑飞%努尔比耶·努尔麦麦提%关亚群
常晞%焦誼%陸劍飛%努爾比耶·努爾麥麥提%關亞群
상희%초의%륙검비%노이비야·노이맥맥제%관아군
腺病毒 36 型%肥胖%颗粒蛋白前体(progranulin)%实时荧光定量-聚合酶链反应(real time quantitative-PCR)%巨噬细胞%维吾尔族
腺病毒 36 型%肥胖%顆粒蛋白前體(progranulin)%實時熒光定量-聚閤酶鏈反應(real time quantitative-PCR)%巨噬細胞%維吾爾族
선병독 36 형%비반%과립단백전체(progranulin)%실시형광정량-취합매련반응(real time quantitative-PCR)%거서세포%유오이족
Ad36%obesity%progranulin%real time PCR%macrophages%Uygur
目的：探讨腺病毒36型(Ad36)感染对维吾尔族肥胖患者颗粒蛋白前体(progranulin)表达的调节作用。方法根据肥胖诊断标准将维吾尔族人群样本分为肥胖组(115)与非肥胖组(117),收集血清标本232份,脂肪标本102份。血清中和反应法检测样本血清中的 Ad36抗体。采用实时荧光定量 PCR(real time quantitative-PCR)方法检测研究对象大网膜和皮下的脂肪组织 progranulin 的 mRNA 表达水平,利用酶联免疫吸附实验(ELISA)测定血清progranulin 的蛋白表达水平。免疫组化方法检测脂肪组织巨噬细胞 CD68蛋白表达,了解巨噬细胞浸润情况。结果①肥胖患者 Ad36感染率(54/115,47.0%)明显高于非肥胖者(38/117,32.5%),差异有统计学意义(P <0.05)。②Ad36感染的肥胖组血清 progranulin 的 蛋 白 表达水平 (408.45±156.92)显著高于非肥胖组(326.11±158.60),差异有统计学意义(P <0.05)；两组的腹部大网膜、皮下脂肪组织 progranulin mRNA 表达水平差异无统计学意义(P 均>0.05)。③Ad36感染的肥胖组巨噬细胞浸润(14730.16±2227.39)明显高于非肥胖组(10786.50±2772.80),差异有统计学意义(P <0.05)。结论Ad36感染可能与维吾尔族肥胖发生有关,其机制可能为通过调节血清 progranulin 的蛋白表达水平而实现。
目的：探討腺病毒36型(Ad36)感染對維吾爾族肥胖患者顆粒蛋白前體(progranulin)錶達的調節作用。方法根據肥胖診斷標準將維吾爾族人群樣本分為肥胖組(115)與非肥胖組(117),收集血清標本232份,脂肪標本102份。血清中和反應法檢測樣本血清中的 Ad36抗體。採用實時熒光定量 PCR(real time quantitative-PCR)方法檢測研究對象大網膜和皮下的脂肪組織 progranulin 的 mRNA 錶達水平,利用酶聯免疫吸附實驗(ELISA)測定血清progranulin 的蛋白錶達水平。免疫組化方法檢測脂肪組織巨噬細胞 CD68蛋白錶達,瞭解巨噬細胞浸潤情況。結果①肥胖患者 Ad36感染率(54/115,47.0%)明顯高于非肥胖者(38/117,32.5%),差異有統計學意義(P <0.05)。②Ad36感染的肥胖組血清 progranulin 的 蛋 白 錶達水平 (408.45±156.92)顯著高于非肥胖組(326.11±158.60),差異有統計學意義(P <0.05)；兩組的腹部大網膜、皮下脂肪組織 progranulin mRNA 錶達水平差異無統計學意義(P 均>0.05)。③Ad36感染的肥胖組巨噬細胞浸潤(14730.16±2227.39)明顯高于非肥胖組(10786.50±2772.80),差異有統計學意義(P <0.05)。結論Ad36感染可能與維吾爾族肥胖髮生有關,其機製可能為通過調節血清 progranulin 的蛋白錶達水平而實現。
목적：탐토선병독36형(Ad36)감염대유오이족비반환자과립단백전체(progranulin)표체적조절작용。방법근거비반진단표준장유오이족인군양본분위비반조(115)여비비반조(117),수집혈청표본232빈,지방표본102빈。혈청중화반응법검측양본혈청중적 Ad36항체。채용실시형광정량 PCR(real time quantitative-PCR)방법검측연구대상대망막화피하적지방조직 progranulin 적 mRNA 표체수평,이용매련면역흡부실험(ELISA)측정혈청progranulin 적단백표체수평。면역조화방법검측지방조직거서세포 CD68단백표체,료해거서세포침윤정황。결과①비반환자 Ad36감염솔(54/115,47.0%)명현고우비비반자(38/117,32.5%),차이유통계학의의(P <0.05)。②Ad36감염적비반조혈청 progranulin 적 단 백 표체수평 (408.45±156.92)현저고우비비반조(326.11±158.60),차이유통계학의의(P <0.05)；량조적복부대망막、피하지방조직 progranulin mRNA 표체수평차이무통계학의의(P 균>0.05)。③Ad36감염적비반조거서세포침윤(14730.16±2227.39)명현고우비비반조(10786.50±2772.80),차이유통계학의의(P <0.05)。결론Ad36감염가능여유오이족비반발생유관,기궤제가능위통과조절혈청 progranulin 적단백표체수평이실현。
ABSTRACT:Objective To investigate the regulation of adenovirus type 36 infection on precursor protein particles progranulin expression in Uygur obese patients.Methods Based on the diagnostic criteria of obesity,the samples were divided into obese group and non-obese group.Serum neutralization test was used to detect the antibody of Ad36.The progranulin mRNA expressions in abdominal omental and subcutaneous adipose tissues were detected by real-time quantitative PCR.ELISA method was used to determine serum progranulin protein levels. CD68 protein expression of macrophage was detected by immunohistochemistry. Results ① In the Uygur population of our study,compared with that in the non-obese group (38/1 1 7,32.5%),the number of obese patients (54/1 1 5,47.0%)infected with Ad36 was significantly higher than that in non-obese group (P <0.05 ).② Serum progranulin was significantly increased in the Ad36-infected obese group (408.45±1 56.92)than in non-obese group (326.1 1±1 58.60)(P <0.05).The mRNA expression of progranulin did not differ between the two groups.③ The macrophage infiltration was significantly higher in the Ad36-infected obese group (14 730.1 6 ± 2 227.39 )than in non-obese group (10 786.50 ± 2 772.80 )(P < 0.05 ).Conclusion Ad36 infection may be associated with the occurrence of obesity in Uygur population,and adenovirus type 36 infection may regulate the expression of serum progranulin at the protein level.
