CAJ | 학술논문

目的：探讨激素性股骨头坏死中 miRNA 的差异性表达变化。方法取激素性股骨头坏死的坏死区域骨组织为实验组,其股骨颈基底部骨组织为对照组；分别提取总 RNA 并进行质检；采用 miRNA 芯片技术检测 miRNAs 的表达并对其表达谱进行分析；运用实时定量 PCR 对差异明显的 miR-125a-3p 和 miR-17-5p 进行验证。结果对激素性股骨头坏死中 miRNA 表达谱进行差异性分析。和对照组相比,实验组中倍数大于2倍,P <0.05的 miRNA 共有11个。8个 miRNA 表达上调,3个表达下调。实时定量 PCR 证实 miR-125a-3p 在坏死标本中高表达,而 miR-17-5p 呈现低表达,与 miRNA 芯片结果一致。结论激素性股骨头坏死区域骨组织与对照组相比 miRNAs 表达发生明显变化,以 miR-125a-3p 和 miR-17-5p 差异最为明显。这表明了它们最有可能参与了激素性股骨头坏死病理过程的调控。
목적：탐토격소성고골두배사중 miRNA 적차이성표체변화。방법취격소성고골두배사적배사구역골조직위실험조,기고골경기저부골조직위대조조；분별제취총 RNA 병진행질검；채용 miRNA 심편기술검측 miRNAs 적표체병대기표체보진행분석；운용실시정량 PCR 대차이명현적 miR-125a-3p 화 miR-17-5p 진행험증。결과대격소성고골두배사중 miRNA 표체보진행차이성분석。화대조조상비,실험조중배수대우2배,P <0.05적 miRNA 공유11개。8개 miRNA 표체상조,3개표체하조。실시정량 PCR 증실 miR-125a-3p 재배사표본중고표체,이 miR-17-5p 정현저표체,여 miRNA 심편결과일치。결론격소성고골두배사구역골조직여대조조상비 miRNAs 표체발생명현변화,이 miR-125a-3p 화 miR-17-5p 차이최위명현。저표명료타문최유가능삼여료격소성고골두배사병리과정적조공。
ABSTRACT:Objective To explore the differential expression of microRNAs (miRNAs)in steroid-induced osteonecrosis (ON).Methods Bone tissues were selected from patients with steroid-induced ON between the necrotic zone of femoral heads and its femoral neck to analyze the miRNA expression profile using the microarray. The most differentially expressed miR-125a-3p and miR-1 7-5p in microarray analysis were further confirmed by real-time quantitative PCR.Results According to the microarray screening,8 miRNAs were upregulated and 3 miRNAs were downregulated in the necrotic zone of femoral heads samples (Fold>2,P <0.05 ).Results of real-time PCR revealed that miR-125a-3p was upregulated and miR-1 7-5p was downregulated in the necrotic zone of femoral heads samples,which were in agreement with the microarray data.Conclusion MiRNA’s differential expression profile of human steroid-induced ON samples was obtained.Among the differentially expressed miRNAs, miR-125a-3p and miR-1 7-5p are the most apparently differentially expressed miRNAs,which may be related to the pathogenesis and development of steroid-induced ON.