CAJ | 학술논문

目的：探讨趋化因子受体 CX3CR1在重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠模型中的表达,判断其能否作为 SAP 发生的预测指标。方法40只 Sprague-Dawley 大鼠随机分为假手术组(sham operation,SO)(n =20)和 SAP 模型组(n=20),分别于制模后6、12、24、48 h 处死大鼠,应用酶联免疫吸附试验(enzyme-linked immu-nosorbent assay,ELISA)法检测 SAP 大鼠血清中 CX3CR1的变化,同时应用免疫组织化学法检测 SAP 大鼠胰腺、肺脏及肾脏组织中 CX3CR1蛋白的表达。结果大鼠 SAP 制模成功后,血清中 CX3CR1的表达逐渐升高,24 h 达最高(542.4 pg/mL),明显高于 SO 组(P <0.05)。大鼠 SAP 组的胰腺、肺脏和肾脏组织中均有 CX3CR1的表达,且明显高于 SO 组,其中胰腺和肺脏组织中 CX3CR1表达在制模24 h 达高峰,肾脏组织中 CX3CR1表达在制模48 h 达高峰。结论趋化因子受体 CX3CR1可能为预测 SAP 严重程度的有效指标之一,值得进一步深入研究。
목적：탐토추화인자수체 CX3CR1재중증급성이선염(severe acute pancreatitis,SAP)대서모형중적표체,판단기능부작위 SAP 발생적예측지표。방법40지 Sprague-Dawley 대서수궤분위가수술조(sham operation,SO)(n =20)화 SAP 모형조(n=20),분별우제모후6、12、24、48 h 처사대서,응용매련면역흡부시험(enzyme-linked immu-nosorbent assay,ELISA)법검측 SAP 대서혈청중 CX3CR1적변화,동시응용면역조직화학법검측 SAP 대서이선、폐장급신장조직중 CX3CR1단백적표체。결과대서 SAP 제모성공후,혈청중 CX3CR1적표체축점승고,24 h 체최고(542.4 pg/mL),명현고우 SO 조(P <0.05)。대서 SAP 조적이선、폐장화신장조직중균유 CX3CR1적표체,차명현고우 SO 조,기중이선화폐장조직중 CX3CR1표체재제모24 h 체고봉,신장조직중 CX3CR1표체재제모48 h 체고봉。결론추화인자수체 CX3CR1가능위예측 SAP 엄중정도적유효지표지일,치득진일보심입연구。
ABSTRACT:Objective To explore the expression of chemokine receptor CX3CR1 in severe acute pancreatitis (SAP)rats model and to estimate whether it can be a potential predictor of SAP.Methods Forty Sprague-Dawley rats were randomly divided into sham-operation (SO)group (n =20)and SAP group (n =20).Rats were killed at 6 h,12 h,24 h and 48 h after model induction.The serum level of CX3CR1 was measured by enzyme-linked immunosorbent assay (ELISA).The expression of CX3CR1 protein in the pancreas,lung and kidney were detected by immunohistochemistry.Results The serum level of CX3CR1 in SAP rats increased gradually after model induction and reached the peak at 24 h (542.4 pg/mL),which were significantly higher than those in SO group (P <0.05).The expression of CX3CR1 was found in the pancreas,lung and kidney tissues of SAP rats and higher than that in SO group.Meanwhile,the expression of CX3CR1 reached the peak at 24 h in the pancreas and lung and at 48 h in the kidney.Conclusion This study suggests that the chemokine receptor CX3CR1 may be one effective index for predicting the severity of acute pancreatitis and deserves further research.