CAJ | 학술논문

目的：高胆固醇饲料(HCD)诱导家兔动脉粥样硬化和高脂血症，分析家兔血浆一氧化氮(NO)水平与动脉粥样硬化损伤之间的关系。方法20只雄性日本大耳白家兔随机分为对照组和实验组，分别饲喂正常饲料或 HCD 16周，检测血脂水平，分离主动脉树，苏丹Ⅳ染色，分析动脉粥样斑块面积。苏木精伊红染色、范吉尔森弹性纤维染色以及免疫组织化学染色方法分析主动脉弓部斑块的组织学特征，检测血浆 NO 水平，并与动脉粥样硬化斑块面积以及斑块中巨噬细胞的表达量进行相关性分析。结果与对照组相比，实验组家兔血浆总胆固醇、甘油三酯、低密度脂蛋白胆固醇及 NO 水平均显著升高。实验组家兔主动脉血管内壁出现大量动脉粥样硬化斑块。动脉粥样硬化斑块面积大小及斑块内巨噬细胞表达量与血浆中 NO 水平呈正相关。结论在 HCD 诱导的家兔动脉粥样硬化斑块中，斑块面积大小及斑块中巨噬细胞表达量均与血浆 NO 有相关性。这提示血浆 NO 水平可能与动脉粥样硬化初期发生和发展相关。
목적：고담고순사료(HCD)유도가토동맥죽양경화화고지혈증，분석가토혈장일양화담(NO)수평여동맥죽양경화손상지간적관계。방법20지웅성일본대이백가토수궤분위대조조화실험조，분별사위정상사료혹 HCD 16주，검측혈지수평，분리주동맥수，소단Ⅳ염색，분석동맥죽양반괴면적。소목정이홍염색、범길이삼탄성섬유염색이급면역조직화학염색방법분석주동맥궁부반괴적조직학특정，검측혈장 NO 수평，병여동맥죽양경화반괴면적이급반괴중거서세포적표체량진행상관성분석。결과여대조조상비，실험조가토혈장총담고순、감유삼지、저밀도지단백담고순급 NO 수평균현저승고。실험조가토주동맥혈관내벽출현대량동맥죽양경화반괴。동맥죽양경화반괴면적대소급반괴내거서세포표체량여혈장중 NO 수평정정상관。결론재 HCD 유도적가토동맥죽양경화반괴중，반괴면적대소급반괴중거서세포표체량균여혈장 NO 유상관성。저제시혈장 NO 수평가능여동맥죽양경화초기발생화발전상관。
ABSTRACT:Objective To analyze the correlation between plasma nitric oxide (NO)level and atherosclerotic lesion in high cholesterol-fed (HCD ) rabbits.Methods Twenty male Japanese white rabbits were divided randomly into two groups,which were fed with normal diet (control group,n =10)or HCD (experimental group, n =10 )for 1 6 weeks.At the end of the experiment,plasma lipid and NO levels were measured.The gross atherosclerotic lesions in each group were detected by Sudan IV staining while intimal lesion area was measured by hematoxylin/eosin (HE)and elastica van gieson (EVG).Moreover,the macrophages (MΦ)and smooth muscle cells (SMC)were detected by immunohistochemical staining.The correlation analysis was made to reveal the relationship between atherosclerotic lesions and plasma NO level.Results Compared with those in control group, the total cholesterol (TC),triglyceride (TG),low-density lipoprotein cholesterol (LDL-C)and NO levels all increased significantly in experimental group.Atherosclerotic lesions appeared on the vascular wall in the latter group.The area of atherosclerotic lesions and MΦ in the plaque had a positive association with plasma NO level. Conclusion There is a relationship between plasma NO level and the size of HCD-induced atherosclerotic lesions in rabbits.Meanwhile,the MΦ positive area in the atherosclerotic plaque is also associated with plasma NO level in cholesterol-fed rabbits,suggesting that plasma NO level may be associated with the occurrence and progress of early atherosclerosis.