CAJ | 학술논문

目的：研究细胞交互作用对细胞多能性的调控作用。方法：建立牙乳头（DPCs）和牙囊细胞（DFCs）体外共培养模型，二者单独培养的细胞为对照组。采用细胞计数、β－gal 染色检测细胞生长和衰老情况；免疫荧光染色检测共培养条件下多能性相关因子 Oct－4、Sox2和 c－Myc 的表达变化；通过 ALP 活性测试检测矿化诱导后的 DPCs 和 DFCs 的矿化能力。结果：共培养组的 DPCs 和 DFCs 的细胞数明显高于对照组（P ＜0．05）；培养至第7代时，共培养组的 DPCs、DFCs 中与衰老相关的 SA－b－gal 表达较对照组明显减弱（P ＜0．05）；Oct－4、Sox2和 c－Myc 在共培养组 DPCs 和 DFCs 中的表达明显强于对照组；共培养组的 DPCs 和 DFCs 经矿化诱导14、21 d 后，其 ALP 活性均显著高于对照组（P ＜0．05）。结论：DPCs 和 DFCs 可通过体外交互作用抑制细胞衰老、促进细胞的多能性相关因子的表达、提高细胞的矿化能力。
목적：연구세포교호작용대세포다능성적조공작용。방법：건립아유두（DPCs）화아낭세포（DFCs）체외공배양모형，이자단독배양적세포위대조조。채용세포계수、β－gal 염색검측세포생장화쇠로정황；면역형광염색검측공배양조건하다능성상관인자 Oct－4、Sox2화 c－Myc 적표체변화；통과 ALP 활성측시검측광화유도후적 DPCs 화 DFCs 적광화능력。결과：공배양조적 DPCs 화 DFCs 적세포수명현고우대조조（P ＜0．05）；배양지제7대시，공배양조적 DPCs、DFCs 중여쇠로상관적 SA－b－gal 표체교대조조명현감약（P ＜0．05）；Oct－4、Sox2화 c－Myc 재공배양조 DPCs 화 DFCs 중적표체명현강우대조조；공배양조적 DPCs 화 DFCs 경광화유도14、21 d 후，기 ALP 활성균현저고우대조조（P ＜0．05）。결론：DPCs 화 DFCs 가통과체외교호작용억제세포쇠로、촉진세포적다능성상관인자적표체、제고세포적광화능력。
AIM:To investigate the effect of cell-cell interaction of dental papilla cells (DPCs)and dental follicle cells (DFCs)on the pluripotency of the cells.METHODS:An in vitro cell-cell co-culture system of DPCs and DFCs was established.Cell growth,cell senescence and the expression of Oct-4,Sox2 and c-Myc were investiga-ted by cell counting,β-gal staining and immunohistochemical staining.The odontogenic differentiation capability of DPCs and DFCs was evaluated by ALP activity assay after 0,7,14,and 21 d of osteogenic induction.RESULTS:Under co-culture condition,cell growth of DPCs and DFCs was promoted,and cell senescence of DPCs and DFCs was inhibited.Expression of Oct-4,Sox2 and c-Myc was significantly elevated in both cells on day 5 after coculture.ALP activity was significantly upregulated in both cells after 14d and 21d of odontogenic induction.CONCLUSION:Opti-mized microenvironment with cell-cell communication may enhance the pluripotency potential of DPCs and DFCs.