CAJ | 학술논문

目的：研究在机械力作用下人牙周膜细胞（hPDLCs）内 Periositn（PN）mRNA 和蛋白的表达变化。方法：采用组织块酶消化法培养 hPDLCs，经鉴定后将第4代细胞随机分为4个实验组和1个对照组（非加力组），4个实验组分别采用 Flexcell－5000 Tension System 应力加载系统对 hPDLCs 加载6、12、24、48 h 的周期性张应力；然后采用 qRT－PCR、Western blot 分别检测各组 hPDLCs 中 PN mRNA 和蛋白的表达变化。结果：在正常 hPDLCs 中表达 PN mRNA 和蛋白；与对照组相比，加力6 h 后，PN mRNA 和蛋白的表达水平均明显降低（P ＜0．05）；加力12 h 后，PN mRNA 和蛋白的表达均逐渐升高，并持续至24 h 达最高水平（P ＜0．05）；而在加力48 h 时，PN mRNA 和蛋白表达均明显下降，并恢复至对照组的表达水平（P ＞0．05）。结论：机械力可诱导hPDLCs 中 PN 的表达增强及活化，且具有时间依赖性；提示 PN 可能参与了细胞内生物力学信号的转导。
목적：연구재궤계력작용하인아주막세포（hPDLCs）내 Periositn（PN）mRNA 화단백적표체변화。방법：채용조직괴매소화법배양 hPDLCs，경감정후장제4대세포수궤분위4개실험조화1개대조조（비가력조），4개실험조분별채용 Flexcell－5000 Tension System 응력가재계통대 hPDLCs 가재6、12、24、48 h 적주기성장응력；연후채용 qRT－PCR、Western blot 분별검측각조 hPDLCs 중 PN mRNA 화단백적표체변화。결과：재정상 hPDLCs 중표체 PN mRNA 화단백；여대조조상비，가력6 h 후，PN mRNA 화단백적표체수평균명현강저（P ＜0．05）；가력12 h 후，PN mRNA 화단백적표체균축점승고，병지속지24 h 체최고수평（P ＜0．05）；이재가력48 h 시，PN mRNA 화단백표체균명현하강，병회복지대조조적표체수평（P ＞0．05）。결론：궤계력가유도hPDLCs 중 PN 적표체증강급활화，차구유시간의뢰성；제시 PN 가능삼여료세포내생물역학신호적전도。
AIM:To observe the effects of cyclic stretch on the expression of periositn(PN)in human perio-dontal ligament cells(hPDLCs).METHODS:Primary hPDLCs were cultured by tissue block enzymolytic method. Cells were subjected to cyclic stretch by Flexcell FX-5000T system at 0.5 Hz of 10% elongation for 6,12,24 and 48 hours respectively.The mRNA and protein expression of PN were measured by qRT-PCR and western blot respective-ly.RESULTS:PN mRNA and protein were positively expressed in control hPDLCs.PN mRNA and protein were de-creased after 6-hour cyclic stretch,then increased after 12-hour cyclic stretch and reached to the highest level after 24-hour treatment(P <0.05).After 48 hour treatment,PN expression began to decline,consistent with the expres-sion level of the control cells.CONCLUSION:Cyclic stretch may change the expression level of PN in hPDLCs in a time-dependent manner.PN may plays an important role in cell signal transduction of mechanical strain.