中国癌症杂志
中國癌癥雜誌
중국암증잡지
CHINA ONCOLOGY
2015年
2期
112-118
,共7页
胰腺神经内分泌肿瘤%DJ-1%免疫组织化学%生存分析
胰腺神經內分泌腫瘤%DJ-1%免疫組織化學%生存分析
이선신경내분비종류%DJ-1%면역조직화학%생존분석
Pancreatic neuroendocrine neoplasms%DJ-1%Immunohistochemistry%Survival analysis
背景与目的：胰腺神经内分泌肿瘤(pancreatic neuroendocrine neoplasms，PNENs)症状复杂多样，易发生误诊和漏诊。本研究旨在探讨PNENs中DJ-1蛋白表达水平及其在病程进展及预后中的临床意义。方法：采用ELISA法检测16例PNENs及25例健康人血清中DJ-1蛋白的水平，采用免疫组织化学法检测了78例PNENs病理组织中DJ-1蛋白的表达情况。结果：PNENs患者血清中DJ-1蛋白水平显著高于健康人(36.19±6.71 vs 24.68±5.94 ng/mL；P＜0.001)；53.8%的PNENs组织中DJ-1蛋白呈阳性表达(42/78)；PNENs组织中DJ-1表达水平与淋巴结转移(P=0.033)、远处转移(P=0.017)、TNM分期(P=0.012)、以及病理分级(P=0.049)呈显著正相关；作为非独立风险因素，DJ-1阳性表达与PNENs患者OS(P=0.003)及DFS(P=0.018)缩短均显著相关。结论：PNENs存在DJ-1蛋白高表达，且与肿瘤的侵袭转移、病程进展及不良预后相关。
揹景與目的：胰腺神經內分泌腫瘤(pancreatic neuroendocrine neoplasms，PNENs)癥狀複雜多樣，易髮生誤診和漏診。本研究旨在探討PNENs中DJ-1蛋白錶達水平及其在病程進展及預後中的臨床意義。方法：採用ELISA法檢測16例PNENs及25例健康人血清中DJ-1蛋白的水平，採用免疫組織化學法檢測瞭78例PNENs病理組織中DJ-1蛋白的錶達情況。結果：PNENs患者血清中DJ-1蛋白水平顯著高于健康人(36.19±6.71 vs 24.68±5.94 ng/mL；P＜0.001)；53.8%的PNENs組織中DJ-1蛋白呈暘性錶達(42/78)；PNENs組織中DJ-1錶達水平與淋巴結轉移(P=0.033)、遠處轉移(P=0.017)、TNM分期(P=0.012)、以及病理分級(P=0.049)呈顯著正相關；作為非獨立風險因素，DJ-1暘性錶達與PNENs患者OS(P=0.003)及DFS(P=0.018)縮短均顯著相關。結論：PNENs存在DJ-1蛋白高錶達，且與腫瘤的侵襲轉移、病程進展及不良預後相關。
배경여목적：이선신경내분비종류(pancreatic neuroendocrine neoplasms，PNENs)증상복잡다양，역발생오진화루진。본연구지재탐토PNENs중DJ-1단백표체수평급기재병정진전급예후중적림상의의。방법：채용ELISA법검측16례PNENs급25례건강인혈청중DJ-1단백적수평，채용면역조직화학법검측료78례PNENs병리조직중DJ-1단백적표체정황。결과：PNENs환자혈청중DJ-1단백수평현저고우건강인(36.19±6.71 vs 24.68±5.94 ng/mL；P＜0.001)；53.8%적PNENs조직중DJ-1단백정양성표체(42/78)；PNENs조직중DJ-1표체수평여림파결전이(P=0.033)、원처전이(P=0.017)、TNM분기(P=0.012)、이급병리분급(P=0.049)정현저정상관；작위비독립풍험인소，DJ-1양성표체여PNENs환자OS(P=0.003)급DFS(P=0.018)축단균현저상관。결론：PNENs존재DJ-1단백고표체，차여종류적침습전이、병정진전급불량예후상관。
Background and purpose:The symptoms of pancreatic neuroendocrine neoplasms (PNENs) are complicated, that might lead to misdiagnosis. This study aimed to detect the expression level of DJ-1 in PNENs and explore the clinical significance of DJ-1 in PNENs.Methods:DJ-1 protien levels in the serum of 16 cases of PNENs patients and 25 cases of healthy persons were detected by ELISA analysis; The expressions of DJ-1 in 78 cases of PNENs tissues were detected by immunohistochemical staining.Results:The DJ-1 level in the serum of PNENs patients was signiifcantly higher than healthy persons (36.19±6.71vs 24.68±5.94 ng/mL;P<0.001);53.8% (42/78) cases of PNENs tissues showed DJ-1 positive staining; DJ-1 expression level in PNENs tissues had signiifcantly positive correlation with lymph node metastasis (P=0.033), distant metastasis (P=0.017), TNM stage (P=0.012), and pathology grade (P=0.049); As a dependent risk factor, DJ-1 expression was signiifcantly associated with shorter OS (P=0.003) and DFS (P=0.018) of PNENs patients.Conclusion:High expression of DJ-1 protein correlated with invasion and metastasis, disease progression, and poor prognosis in PNEN.
