CAJ | 학술논문

目的：探讨自噬/溶酶体凋亡信号在甘氨鹅脱氧胆酸（GCDC）处理的肺泡 II 型上皮细胞（AECII）中的变化及作用。方法甘氨鹅脱氧酶处理肺泡上皮 A549细胞后，检测自噬途径的标志蛋白LC3II浓度、细胞内钙浓度及溶酶体酶PH值。结果甘氨鹅脱氧胆酸抑制A549自噬启动蛋白LC3II的表达、增加A549胞内Ca2+浓度、降低溶酶体功能。结论甘氨鹅脱氧胆酸会影响肺泡上皮细胞自噬相关蛋白表达，损伤溶酶体活性和通透性，可能导致细胞凋亡。
목적：탐토자서/용매체조망신호재감안아탈양담산（GCDC）처리적폐포 II 형상피세포（AECII）중적변화급작용。방법감안아탈양매처리폐포상피 A549세포후，검측자서도경적표지단백LC3II농도、세포내개농도급용매체매PH치。결과감안아탈양담산억제A549자서계동단백LC3II적표체、증가A549포내Ca2+농도、강저용매체공능。결론감안아탈양담산회영향폐포상피세포자서상관단백표체，손상용매체활성화통투성，가능도치세포조망。
Objective To explore the changes and role of autophagy/lysosomal apoptotic signal induced by Glycocholic CDCA (GCDC) in type II alveolar epithelial cells (AECII). Methods A549 alveolar epithelial cells were treated with DCDC. Protein LC3II, intracellular calcium concentration and PH value of lysosomal enzymes were detected. Results LC3II expression was inhibited by GCDC in A549. Intracellular calcium concentration was increased, and lysosomal function value was decreased in A549. Conclusion GCDC is considered to affect the expression of alveolar epithelial cell autophagy, and damage lysosomal activity and permeability which could lead to apoptosis.