CAJ | 학술논문

目的：探讨叉头状转录因子 O1(Forkhead transcription factor,FoxO1)过表达对糖尿病大鼠肾小球系膜细胞(mesangial cell,MCs)增殖的影响及其机制。方法构建空慢病毒载体(LV-pSC-GFP)、大鼠组成性激活突变型 FoxO1慢病毒载体(LV-CA-FoxO1)。建立糖尿病大鼠模型,造模成功后随机分为糖尿病组(DM 组),糖尿病空病毒转染组(NC 组),糖尿病 FoxO1过表达转染组(CA 组),选健康同龄雄性 SD大鼠做正常对照(NG 组)。然后将慢病毒分别一次性靶向注入对应组糖尿病大鼠肾脏皮质内,正常组注射等体积生理盐水,分别于转染后2周,4周,8周时检测大鼠体重、血糖、血肌酐、尿素氮、24 h 尿蛋白及尿白蛋白定量。处死动物后计算肾重指数,留取肾脏组织制备冰冻及光镜和电镜切片。实时荧光定量 PCR 分别检测各组大鼠肾皮质 FoxO1,细胞周期蛋白依赖性激酶抑制1B( cyclin-dependent kinase inhibitor 1B, p27Kip1)的 mRNA 水平,Western 印迹法检测 FoxO1、磷酸化 FoxO1(p-FoxO1)、p27Kip1的蛋白表达。结果倒置荧光显微镜下观察到转染组多量绿色荧光蛋白表达；光镜及电镜结果显示各时间段 CA 组肾脏病变较DM 组明显改善。与 DM 组相比,各时间段 CA 组 FoxO1,p27Kip1 mRNA 及蛋白水平显著升高(P<0.05), p-FoxO1蛋白表达与 DM 组无差异(P>0.05),但 p-FoxO1/ FoxO1比值明显降低(P<0.05)。 NC 组与 DM 组比较各指标之间差异无统计学意义(P>0.05)。结论靶向性调节糖尿病大鼠肾皮质中 FoxO1的表达,能够显著改善糖尿病状态下系膜细胞的增殖,机制可能是通过上调其靶基因 p27Kip1的表达,延缓糖尿病肾病的发生发展。
목적：탐토차두상전록인자 O1(Forkhead transcription factor,FoxO1)과표체대당뇨병대서신소구계막세포(mesangial cell,MCs)증식적영향급기궤제。방법구건공만병독재체(LV-pSC-GFP)、대서조성성격활돌변형 FoxO1만병독재체(LV-CA-FoxO1)。건립당뇨병대서모형,조모성공후수궤분위당뇨병조(DM 조),당뇨병공병독전염조(NC 조),당뇨병 FoxO1과표체전염조(CA 조),선건강동령웅성 SD대서주정상대조(NG 조)。연후장만병독분별일차성파향주입대응조당뇨병대서신장피질내,정상조주사등체적생리염수,분별우전염후2주,4주,8주시검측대서체중、혈당、혈기항、뇨소담、24 h 뇨단백급뇨백단백정량。처사동물후계산신중지수,류취신장조직제비빙동급광경화전경절편。실시형광정량 PCR 분별검측각조대서신피질 FoxO1,세포주기단백의뢰성격매억제1B( cyclin-dependent kinase inhibitor 1B, p27Kip1)적 mRNA 수평,Western 인적법검측 FoxO1、린산화 FoxO1(p-FoxO1)、p27Kip1적단백표체。결과도치형광현미경하관찰도전염조다량록색형광단백표체；광경급전경결과현시각시간단 CA 조신장병변교DM 조명현개선。여 DM 조상비,각시간단 CA 조 FoxO1,p27Kip1 mRNA 급단백수평현저승고(P<0.05), p-FoxO1단백표체여 DM 조무차이(P>0.05),단 p-FoxO1/ FoxO1비치명현강저(P<0.05)。 NC 조여 DM 조비교각지표지간차이무통계학의의(P>0.05)。결론파향성조절당뇨병대서신피질중 FoxO1적표체,능구현저개선당뇨병상태하계막세포적증식,궤제가능시통과상조기파기인 p27Kip1적표체,연완당뇨병신병적발생발전。
Objective To study the role and molecular mechanism of forkhead transcription factor O1 (FoxO1) on proliferation of mesangial cells( MCs) in diabetic rats. Methods Empty lentiviral vector( LV-pSC-GFP) and the constitutively active FoxO1 lentiviral vector(LV-CA-FoxO1) were constructed. Diabetic rat model was established and rats were divided into diabetes group(DM group), diabetes with LV-pSC-GFP group(NC group), and diabetes with LV-CA-FoxO1 group(CA group). The normal SD rats of the same age were considered as the normal control group(NG group). The lentiviral vector was injected into the renal cortex of diabetic rats in corresponding groups. Body weight, blood glucose, 24 h urinary protein, urine albumin, serum creatinine, and blood urea nitrogen was detected at the end of 2 weeks, 4 weeks, and 8 weeks. The ratio of kidney weight/ body weight was counted and the renal cortex was reserved for light microscopy, electron microscopy and frozen section after rats were sacrificed in different groups. The mRNA level of FoxO1 and p27Kip1 were detected by real-time PCR. The protein expressions of FoxO1, p-FoxO1, and p27Kip1 were tested by Western blotting. Results The renal pathological changes were obviously ameliorated in CA group. Compared with DM group, the mRNA and protein expression of FoxO1 and p27Kip1 were significantly increased in CA group (P<0. 05), whereas there was no difference in the expression of p-FoxO1 protein(P > 0. 05). The p-FoxO1 / FoxO1 ratio was decreased ( P < 0. 05). All indexes had not reached statistical difference between NC group and DM group(P>0. 05). Conclusion Overexpression of FoxO1 in kidneys of diabetic rats can inhibit the proliferation of mesangial cells, and may through up-regulating the expression of p27Kip1 delay the progression of diabetic nephropathy.