CAJ | 학술논문

目的：建立款冬花总生物碱含量测定方法，并对款冬花生品和炮制品的含量进行比较研究。方法：采用酸性染料比色法，检测波长：417.5 nm。结果：款冬花总生物碱在6.75～135.00μg范围内呈良好的线性关系，回归方程：y=85.228x-0.0401（r=0.9995）。款冬花生品样品中总生物碱平均含量为0.1070 mg·g-1，蜜炙品样品中总生物碱平均含量为0.1633 mg·g-1，甘草炙品样品中总生物碱平均含量为0.0861 mg·g-1。结论：该方法经方法学验证可用于款冬花中总生物碱含量的测定。款冬花生品经炮制后，总生物碱含量发生变化，且炮制方法不同，总生物碱含量变化不同。蜜炙品的总生物碱含量最高，生品次之，甘草炙品最低。
목적：건립관동화총생물감함량측정방법，병대관동화생품화포제품적함량진행비교연구。방법：채용산성염료비색법，검측파장：417.5 nm。결과：관동화총생물감재6.75～135.00μg범위내정량호적선성관계，회귀방정：y=85.228x-0.0401（r=0.9995）。관동화생품양품중총생물감평균함량위0.1070 mg·g-1，밀자품양품중총생물감평균함량위0.1633 mg·g-1，감초자품양품중총생물감평균함량위0.0861 mg·g-1。결론：해방법경방법학험증가용우관동화중총생물감함량적측정。관동화생품경포제후，총생물감함량발생변화，차포제방법불동，총생물감함량변화불동。밀자품적총생물감함량최고，생품차지，감초자품최저。
Objective:To establish the method of determination of total alkaloid content inTussilago farfara, and make a comparative study on total alkaloid content inTussilago farfarabefore and after processing. Methods:The content of total alkaloids was determined by acid dye colorimetry, with UV detection wavelength set at 417.5 nm.Results:The total alkaloid content inTussilago farfara was in good linear in the range of 6.75-135.00 μg, and its regression equation wasy=85.228x-0.0401,r=0.9995. The total alkaloid content was 0.1070 mg·g-1 in crudeTussilago farfara, 0.1633 mg·g-1 inTussilago farfara after honey-fried processing, and 0.0861 mg·g-1 inTussilago farfara after liquorice juice processing.Conclusion:This methodologically validated method can be used for determination of the total alkaloids inTussilago farfara. The content of total alkaloids changed after processing, and it changed with the processing methods simultaneously. The total alkaloid content was the highest inTussilago farfara after honey-fried processing, medium in crudeTussilago farfara and the least after liquorice juice processing.