CAJ | 학술논문

目的：：研究绞股蓝大孔树脂分离物对二苯基苦基苯肼( DPPH)自由基的清除作用。方法：将绞股蓝水煮醇沉的提取物用大孔树脂分离,依次以H2 O,40%乙醇,80%乙醇洗脱,得到相应的分离物；采用DPPH自由基体系法测定其抗氧化活性,并与没食子酸、抗坏血酸进行比较。同时,测定各分离物中总皂苷的相对含量。结果：绞股蓝80%乙醇,40%乙醇,H2 O分离产物对DPPH自由基的半数抑制浓度分别为0.0812,0.0287,0.5410g·L-1。40%乙醇分离物中皂苷的相对含量最高。结论：绞股蓝不同溶剂大孔树脂分离物均有抗氧化活性,其中40%乙醇分离物的抗氧化性最强。预示绞股蓝皂苷是降脂、降糖以及清除自由基的可能活性物质。
목적：：연구교고람대공수지분리물대이분기고기분정( DPPH)자유기적청제작용。방법：장교고람수자순침적제취물용대공수지분리,의차이H2 O,40%을순,80%을순세탈,득도상응적분리물；채용DPPH자유기체계법측정기항양화활성,병여몰식자산、항배혈산진행비교。동시,측정각분리물중총조감적상대함량。결과：교고람80%을순,40%을순,H2 O분리산물대DPPH자유기적반수억제농도분별위0.0812,0.0287,0.5410g·L-1。40%을순분리물중조감적상대함량최고。결론：교고람불동용제대공수지분리물균유항양화활성,기중40%을순분리물적항양화성최강。예시교고람조감시강지、강당이급청제자유기적가능활성물질。
Objective:To study the scavenging activity of macroporous resin isolate of gynostemma pentaphyllum on free radical DPPH. Methods:The extract of gynostemma pentaphyllum via water boiling and precipitation with ethanol was separated by macro-porous resin respectively eluted by water, 40% ethanol and 80% ethanol to obtain the corresponding isolates. The antioxidant activity of the three isolates was determined by free radical DPPH systematic method, and the results were compared with that of gallic acid and ascorbic acid. Results: The 50% inhibitory concentration on free radical DPPH of the three isolates was 0. 081 2, 0. 028 7 and 0. 541 0 g·L-1 ,respectively. The 40% ethanol isolate had the highest saponins concentration. Conclusion:The macroporous resin i-solate with different solvent all shows antioxidant activity and the 40% ethanol isolate has the strongest effect, suggesting saponins may be the active substances for the lipid-lowering, hypoglycemic and free radical scavenging activities.