CAJ | 학술논문

目的：：采用Q-TRAP LC-MS/MS测定中药白及中活性成分militarine、原儿茶酸和咖啡酸的含量。方法：采用Supelco Discovery C-18(150 mm ×2.1 mm,3μm)色谱柱,以乙腈和水(含体积比0.1%甲酸)为流动相,梯度洗脱,流速为0.25 ml· min-1,AB Sciex 4000 Q-TRAP型质谱仪多重反应监测( MRM)扫描模式对白及对照药材和白及样品分别进行检测。结果：白及对照药材中militarine、原儿茶酸和咖啡酸的含量分别为1.1675,0.0626,0.0010 mg·g-1.白及供试品药材中militarine、原儿茶酸和咖啡酸的含量分别为0.7088,0.0011,0.0004 mg·g-1(n=3)。结论：该方法简便、准确、重现性好,可用于对中药白及中militarine、原儿茶酸和咖啡酸这三个成分进行定量分析。
목적：：채용Q-TRAP LC-MS/MS측정중약백급중활성성분militarine、원인다산화가배산적함량。방법：채용Supelco Discovery C-18(150 mm ×2.1 mm,3μm)색보주,이을정화수(함체적비0.1%갑산)위류동상,제도세탈,류속위0.25 ml· min-1,AB Sciex 4000 Q-TRAP형질보의다중반응감측( MRM)소묘모식대백급대조약재화백급양품분별진행검측。결과：백급대조약재중militarine、원인다산화가배산적함량분별위1.1675,0.0626,0.0010 mg·g-1.백급공시품약재중militarine、원인다산화가배산적함량분별위0.7088,0.0011,0.0004 mg·g-1(n=3)。결론：해방법간편、준학、중현성호,가용우대중약백급중militarine、원인다산화가배산저삼개성분진행정량분석。
Objective:To develop a Q-TRAP LC-MS/MS method for the content determination of militarine, protocatechuic acid and caffeic acid in Bletilla Striata. Methods:A Supelco Discovery C-18 (150 mm × 2. 1 mm, 3 μm) column was used and the mobile phase was acetonitrile-water containing 0. 1% formic acid (v/v) with gradient elution. The flow rate was 0. 25 ml·min-1. Bletilla Striata contrast medicinal materials and Bletilla Striata samples were detected by AB Sciex 4000 Q-MRM scan mode. Results: The content of militarine, protocatechuic acid and caffeic acid in the control herb of Bletilla Striata was 1. 167 5, 0. 062 6 mg·g-1 and 0. 001 0 mg·g-1 , respectively, and that in Bletilla Striata samples was 0. 708 8, 0. 001 1 mg·g-1 and 0. 000 4 mg·g-1 , respec-tively(n=3). Conclusion:The method is simple, accurate and reproducible. It can be used to quantitatively analyze militarine, pro-tocatechuic acid and caffeic acid in Bletilla Striata.