CAJ | 학술논문

目的：：初步考察5种中药成分黄芩苷、穿心莲内酯、橙皮苷、茶多酚及黄豆苷元在体外对羧酸酯酶1(CES1)和羧酸酯酶2(CES2)活性的影响。方法：采用CES1、CES2特异性探针咪达普利和伊立替康(CPT-11)及其代谢产物咪达普利拉和SN-38,在大鼠肝微粒体孵育体系中初步考察上述5种中药成分抑制两种CESs活性强度。结果：上述5种中药成分在100μmol ·L-1浓度下对CES1、CES2活性均无显著抑制作用(P<0.05)。结论：黄芩苷、穿心莲内酯、橙皮苷、茶多酚及黄豆苷元在体外不影响CESs活性,体内实验有待进一步开展。
목적：：초보고찰5충중약성분황금감、천심련내지、등피감、다다분급황두감원재체외대최산지매1(CES1)화최산지매2(CES2)활성적영향。방법：채용CES1、CES2특이성탐침미체보리화이립체강(CPT-11)급기대사산물미체보리랍화SN-38,재대서간미립체부육체계중초보고찰상술5충중약성분억제량충CESs활성강도。결과：상술5충중약성분재100μmol ·L-1농도하대CES1、CES2활성균무현저억제작용(P<0.05)。결론：황금감、천심련내지、등피감、다다분급황두감원재체외불영향CESs활성,체내실험유대진일보개전。
Objective:To evaluate the inhibitory effect of 5 Chinese herbal medicinal ingredients baicalin, andrographolide, hes-peridin, polyphenols and daidzein on the activity of carboxylesterase 1 (CES1) and carboxylesterase 2 (CES2). Methods: The spe-cial probes respectively for CES1 and CES2, namely imidapril and CPT-11, imidaprilat and SN-38, were used to investigate the poten-tial effect of the above drugs on CES1 and CES2 in rat liver microsomes incubation system. Results:Compared with that in the control group, the activity of CES1 and CES2 was not significantly influenced by the above 5 Chinese herbal medicinal ingredients ( P <0. 05). Conclusion:Baicalin, andrographolide, hesperidin, polyphenols and daidzein exhibit no inhibitory effect against CES1 and CES2, and further studies should be conducted to confirm the effect in vivo.