中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
2期
315-316,357
,共3页
活络消痛片%紫丁香苷%阿魏酸%丹酚酸B%高效液相色谱法
活絡消痛片%紫丁香苷%阿魏痠%丹酚痠B%高效液相色譜法
활락소통편%자정향감%아위산%단분산B%고효액상색보법
Huoluo Xiaotong tablets%Syringin%Ferulic acid%Salvianolic acid B%HPLC
目的：：建立HPLC法同时测定活络消痛片中紫丁香苷、阿魏酸和丹酚酸B的含量。方法：色谱柱为Kromasil C18柱(150 mm ×4.6 mm,5μm),流动相为甲醇-0.2%磷酸溶液,梯度洗脱,流速为1.0 ml·min-1,检测波长为紫丁香苷(265 nm),阿魏酸(321 nm),丹酚酸B(286 nm),柱温为25℃。结果：紫丁香苷、阿魏酸和丹酚酸B分别在64.500~1.032×103μg· ml-1、7.525~120.400μg·ml-1、65.250~1.044×103μg · ml-1范围内与峰面积呈良好的线性关系,平均回收率分别为100.75%、99.56%、99.75%。结论：本方法简便、准确、快速。
目的：：建立HPLC法同時測定活絡消痛片中紫丁香苷、阿魏痠和丹酚痠B的含量。方法：色譜柱為Kromasil C18柱(150 mm ×4.6 mm,5μm),流動相為甲醇-0.2%燐痠溶液,梯度洗脫,流速為1.0 ml·min-1,檢測波長為紫丁香苷(265 nm),阿魏痠(321 nm),丹酚痠B(286 nm),柱溫為25℃。結果：紫丁香苷、阿魏痠和丹酚痠B分彆在64.500~1.032×103μg· ml-1、7.525~120.400μg·ml-1、65.250~1.044×103μg · ml-1範圍內與峰麵積呈良好的線性關繫,平均迴收率分彆為100.75%、99.56%、99.75%。結論：本方法簡便、準確、快速。
목적：：건립HPLC법동시측정활락소통편중자정향감、아위산화단분산B적함량。방법：색보주위Kromasil C18주(150 mm ×4.6 mm,5μm),류동상위갑순-0.2%린산용액,제도세탈,류속위1.0 ml·min-1,검측파장위자정향감(265 nm),아위산(321 nm),단분산B(286 nm),주온위25℃。결과：자정향감、아위산화단분산B분별재64.500~1.032×103μg· ml-1、7.525~120.400μg·ml-1、65.250~1.044×103μg · ml-1범위내여봉면적정량호적선성관계,평균회수솔분별위100.75%、99.56%、99.75%。결론：본방법간편、준학、쾌속。
Objective:To establish a determination method for syringin, ferulic acid and salvianolic acid B in Huoluo Xiaotong tabletsbyHPLC.Methods:ThecolumnwasKromasilC18(150mm×4.6mm,5 μm),themobilephasewasmethanol-0.2% phos-phoric acid solution with gradient elution at the flow rate of 1. 0 ml·min-1 , the detection wavelengths were 265nm for Sgnigin, 321nm for Ferulic acid and 286nm for Salvianolic acid B. The column temperature was 25℃. Results: Syringin, ferulic acid and salvianolic acid B showed good linear relationship within the range of 64. 500-1. 032 × 103 , 7. 525-120. 400, 65. 250-1. 044 × 103 μg·ml-1 with the average recovery of 100. 75%, 99. 56% and 99. 75%, respectively. Conclusion:The method is simple, accurate and rapid.
