中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
2期
312-315
,共4页
宁霄%金绍明%曹进%张庆生
寧霄%金紹明%曹進%張慶生
저소%금소명%조진%장경생
高效液相色谱%香兰素%乳制品%快速测定
高效液相色譜%香蘭素%乳製品%快速測定
고효액상색보%향란소%유제품%쾌속측정
HPLC%Vanillin%Dairy products%Rapid analysis
目的：：建立一种高效液相色谱法测定乳制品中香兰素含量的方法。方法：以18种乳制品为样品,采用C18(250 mm ×4.6 mm,5μm)反相色谱柱,甲醇-水(35∶65)为流动相,流速为1.0 ml·min-1,检测波长为275 nm。结果：结果表明,各种不同基质样品中香兰素检出限为0.450μg·ml-1,浓度在1.990~99.500μg·ml-1的范围内呈线性关系(r=1.0000),低、中、高三水平回收率在96.26%~100.81%之间,RSD在0.15%~2.22%之间。结论：该方法操作简便,结果可靠,可用于乳制品中香兰素含量的快速测定。
目的：：建立一種高效液相色譜法測定乳製品中香蘭素含量的方法。方法：以18種乳製品為樣品,採用C18(250 mm ×4.6 mm,5μm)反相色譜柱,甲醇-水(35∶65)為流動相,流速為1.0 ml·min-1,檢測波長為275 nm。結果：結果錶明,各種不同基質樣品中香蘭素檢齣限為0.450μg·ml-1,濃度在1.990~99.500μg·ml-1的範圍內呈線性關繫(r=1.0000),低、中、高三水平迴收率在96.26%~100.81%之間,RSD在0.15%~2.22%之間。結論：該方法操作簡便,結果可靠,可用于乳製品中香蘭素含量的快速測定。
목적：：건립일충고효액상색보법측정유제품중향란소함량적방법。방법：이18충유제품위양품,채용C18(250 mm ×4.6 mm,5μm)반상색보주,갑순-수(35∶65)위류동상,류속위1.0 ml·min-1,검측파장위275 nm。결과：결과표명,각충불동기질양품중향란소검출한위0.450μg·ml-1,농도재1.990~99.500μg·ml-1적범위내정선성관계(r=1.0000),저、중、고삼수평회수솔재96.26%~100.81%지간,RSD재0.15%~2.22%지간。결론：해방법조작간편,결과가고,가용우유제품중향란소함량적쾌속측정。
Objective:To establish an HPLC method to determine vanillin in 18 dairy products. Methods:The samples were sep-arated on a C18 column (250 mm × 4. 6 mm, 5 μm) with the mobile phase of methanol and water (35∶65) at the flow rate of 1. 0 ml ·min-1 . The detection wavelength was 275 nm. Results:The LOD ( limit of detection) of vanillin was 0. 45 μg·ml-1 . Excellent linearity was obtained within the concentration range of 1.990-99.500 μg·ml-1(r=1.000 0). The average recovery of vanillin in different dairy products with low, medium and high levels varied between 96. 26% and 100. 81% with RSD of 0. 15%-2. 22%. Con-clusion:The method is simple and reproducible, which can be applied in the rapid analysis of vanillin in dairy products.
