CAJ | 학술논문

目的：进行山豆根水提组分对咽喉实热证小鼠的急性毒性研究，以阐释清热解毒类有毒中药“毒性-证候”相关性。方法制备山豆根水提组分样品，建立小鼠咽喉实热证模型，采用经典的小鼠急性毒性实验方法给模型小鼠灌胃山豆根水提组分进行急性毒性实验，连续观察14天，观察其急性毒性症状、体征，记录累积死亡率和小鼠体重变化，计算LD50。结果山豆根水提组分给咽喉实热证模型小鼠灌胃后小鼠出现烦躁、呼吸抑制等急毒症状、体征，LD50及其95%可信限为20.63（17.94~23.92）g·kg-1。结论山豆根水提组分对咽喉实热证模型小鼠的急性毒性明显小于正常动物，毒性症状出现时间延后，毒性症状减轻，LD50值增大。
목적：진행산두근수제조분대인후실열증소서적급성독성연구，이천석청열해독류유독중약“독성-증후”상관성。방법제비산두근수제조분양품，건립소서인후실열증모형，채용경전적소서급성독성실험방법급모형소서관위산두근수제조분진행급성독성실험，련속관찰14천，관찰기급성독성증상、체정，기록루적사망솔화소서체중변화，계산LD50。결과산두근수제조분급인후실열증모형소서관위후소서출현번조、호흡억제등급독증상、체정，LD50급기95%가신한위20.63（17.94~23.92）g·kg-1。결론산두근수제조분대인후실열증모형소서적급성독성명현소우정상동물，독성증상출현시간연후，독성증상감경，LD50치증대。
Objective The acute toxicity of water extract of Sophorae tonkinensis Radix et Rhizome on mice was researched to determine the correlation of "toxicity-syndrome". Methods The sample water extract of Sophorae tonkinensis Radix et Rhizome was administrated to KM mice of the throat excess-heat syndrome models. The LD 50 of Sophorae tonkinensis Radix et Rhizome was tested by the classical methods of acute toxicity. The acute toxic symptoms were observed, the accumulated death number and the weight-changes of live mice were recorded. Results The LD50 and its 95% confidence limits of water extract of Sophorae tonkinensis Radix et Rhizome was 20.63 (17.94~23.92)g·kg-1 in mice. Restlessness, breathlessness and convulsion were the main acute toxic symptoms. Conclusion The acute toxicity of water extract of Sophorae tonkinensis Radix et Rhizome on throat excess-heat syn drome mouse model in mice was significantly less than normal animals. The onset time of toxic symptoms was delayed and relieved, the LD50 value was increased.