CAJ | 학술논문

目的：明确 Dab2在人正常肾上腺组织中的表达及分布,进一步探讨 Dab2在不同类型肾上腺皮质腺瘤组织中的表达情况,以及 Dab2是否可作为原发性醛固酮增多症病理诊断特异分子标记。方法采用免疫组化和实时定量 PCR 的方法检测了10例醛固酮瘤组织、8例皮质醇瘤组织、8例无功能皮质腺瘤组织和6名正常肾上腺组织中 Dab2的表达情况。结果免疫组化结果显示,Dab2在人正常肾上腺皮质球状带中显著高表达；在醛固酮瘤组织中可见散在的成簇状 Dab2中等强度表达区域,在所有的皮质醇瘤和无功能皮质腺瘤组织标本中,均显示较弱的 Dab2染色。 RT-PCR 的结果表明,与正常肾上腺组织相比, Dab2 mRNA 在醛固酮瘤中表达增高(P<0.05)。皮质醇瘤及无功能皮质腺瘤中 Dab2 mRNA 的表达水平较正常肾上腺组织无显著性变化；与各自的瘤周肾上腺组织相比,Dab2 mRNA 在醛固酮瘤的肿瘤组织中表达也增高。结论 Dab2特异高表达于正常人肾上腺组织球状带,Dab2 mRNA 在醛固酮瘤组织中表达明显升高,Dab2蛋白在醛固酮瘤中呈现中等强度表达,Dab2能否作为原发性醛固酮增多症病理诊断的特异分子标记还有待于进一步研究明确。
목적：명학 Dab2재인정상신상선조직중적표체급분포,진일보탐토 Dab2재불동류형신상선피질선류조직중적표체정황,이급 Dab2시부가작위원발성철고동증다증병리진단특이분자표기。방법채용면역조화화실시정량 PCR 적방법검측료10례철고동류조직、8례피질순류조직、8례무공능피질선류조직화6명정상신상선조직중 Dab2적표체정황。결과면역조화결과현시,Dab2재인정상신상선피질구상대중현저고표체；재철고동류조직중가견산재적성족상 Dab2중등강도표체구역,재소유적피질순류화무공능피질선류조직표본중,균현시교약적 Dab2염색。 RT-PCR 적결과표명,여정상신상선조직상비, Dab2 mRNA 재철고동류중표체증고(P<0.05)。피질순류급무공능피질선류중 Dab2 mRNA 적표체수평교정상신상선조직무현저성변화；여각자적류주신상선조직상비,Dab2 mRNA 재철고동류적종류조직중표체야증고。결론 Dab2특이고표체우정상인신상선조직구상대,Dab2 mRNA 재철고동류조직중표체명현승고,Dab2단백재철고동류중정현중등강도표체,Dab2능부작위원발성철고동증다증병리진단적특이분자표기환유대우진일보연구명학。
Objective To determine the expression and distribution of Disabled-2(Dab2) in normal human adrenal glands, and further to study the expression of Dab2 in tissues of different adrenocortical adenomas, and to elucidate whether Dab2 can be a specific molecular marker in the pathology of primary aldosteronism. Methods Real-time PCR and immunohistochemical staining were used to detect Dab2 expression in 10 aldosterone-producing adenoma (APA) samples, 8 cortisol-producing adenoma ( CPA) samples, 8 non-functioning adenoma ( NFA) samples and 6 normal adrenal samples. Results Immunohistochemical staining showed that Dab2 was significantly highly expressed in zona glomerulosa of normal human adrenal glands. Sporadical cluster of ZG cells with moderate Dab2 staining were demonstrated in APAs. In all CPA and NFA tumors, weak dab2 staining was detected. According to the results of real-time PCR, Dab2 mRNA expression was increased significantly in APAs compared with normal adrenal glands. There was no significant difference between normal adrenal glands, CPAs, and NFAs in regard to Dab2 mRNA expression. Compared to nontumor portions, APAs also showed higher Dab2 mRNA expression in the tumor( P<0. 05). Conclusion Dab2 was predominantly localized in zona glomerulosa in normal adrenal gland. Increased Dab2 mRNA expression was detected in APAs compared with normal adrenal glands. Whereas, Dab2 protein expression was just moderate increased in APAs. Weather Dab2 can be a specific molecular marker in the pathology of primary aldosteronism has to be further studied.