CAJ | 학술논문

本文通过对东北粗皮蛙29个样本线粒体DNA控制区和细胞色素b基因部分序列分析，探讨其遗传多样性和种群遗传结构。 PCR扩增和测序结果，获得587bp的线粒体DNA控制区序列和895bp的细胞色素b基因序列。合并线粒体DNA序列分析，共定义14个单倍型，16个变异位点，单倍型多样性（H）为0．704，核苷酸多样性（π）为0．001，平均核苷酸差异数为1．438，表明东北粗皮蛙遗传多样性较低。分子变异分析（AMOVA）结果表明，种群内变异占全部遗传变异的98．13％，群体间没有遗传分化（FST ＝0．0187），因此应加强对东北粗皮蛙野生种群的保护。
본문통과대동북조피와29개양본선립체DNA공제구화세포색소b기인부분서렬분석，탐토기유전다양성화충군유전결구。 PCR확증화측서결과，획득587bp적선립체DNA공제구서렬화895bp적세포색소b기인서렬。합병선립체DNA서렬분석，공정의14개단배형，16개변이위점，단배형다양성（H）위0．704，핵감산다양성（π）위0．001，평균핵감산차이수위1．438，표명동북조피와유전다양성교저。분자변이분석（AMOVA）결과표명，충군내변이점전부유전변이적98．13％，군체간몰유유전분화（FST ＝0．0187），인차응가강대동북조피와야생충군적보호。
The partial sequences of mitochondrial DNA control region and cytochrome b gene from 29 individuals were studied via PCR and sequencing, to investigate the genetic structure and genetic diversity of Rugosa emeljanowi.We obtained 587bp from mitochondrial DNA control region and 895bp from cytochrome b gene.Based on the analysis of coalescent data , 16 polymorphic sites were found and 14 haplotypes were detected .Haplotype diversity, nucleotide diversity and average number of nucleotide differences were 0.704, 0.001 and 1.438, respectively.These results showed that there was a lower genetic diversity among the population of Rugosa emeljanovi.Ac-cording to analysis of molecular variance (AMOVA), the genetic variation within populations was very high , being 98.13 %.The ge-netic differentiation between populations was not significant (FST=0.0187).The results suggested that we need to strengthen the con-servation for populations of Rugosa emeljanovi.