CAJ | 학술논문

目的：：探讨丹皮酚(Pae)对急性心肌梗死(AMI)后心室重构大鼠心肌组织TGF-β1表达的影响。方法：SD大鼠随机分为假手术组、模型组、Pae组和卡托普利组。采用结扎大鼠左冠状动脉前降支的方法建立AMI大鼠模型，Pae组大鼠腹腔注射Pae(16.0mg/kg)，卡托普利组大鼠灌胃给予卡托普利(10.0mg/kg)，连续给药4周。分别检测各组大鼠的左心指数(LVWI)、左室胶原容积分数(CVF)，免疫组化法检测心肌组织TGF-β1蛋白的表达。结果：与模型组大鼠比较，Pae组、卡托普利组大鼠的LVWI、CVF和心肌组织TGF-β1蛋白的表达明显降低(P＜0.01)。结论：Pae改善大鼠心室重构的机制可能与其下调TGF-β1蛋白的表达有关。
목적：：탐토단피분(Pae)대급성심기경사(AMI)후심실중구대서심기조직TGF-β1표체적영향。방법：SD대서수궤분위가수술조、모형조、Pae조화잡탁보리조。채용결찰대서좌관상동맥전강지적방법건립AMI대서모형，Pae조대서복강주사Pae(16.0mg/kg)，잡탁보리조대서관위급여잡탁보리(10.0mg/kg)，련속급약4주。분별검측각조대서적좌심지수(LVWI)、좌실효원용적분수(CVF)，면역조화법검측심기조직TGF-β1단백적표체。결과：여모형조대서비교，Pae조、잡탁보리조대서적LVWI、CVF화심기조직TGF-β1단백적표체명현강저(P＜0.01)。결론：Pae개선대서심실중구적궤제가능여기하조TGF-β1단백적표체유관。
[ABSTRACT]Objective:To observe the effects of paeonol on TGF-β1 expression in myocardial tissue of ventricular remodeling rats after acute myocardial infarction (AMI). Methods:SD rats were randomly divided into sham-operation group, model group, paeonol group and captopril group. The AMI rats model was made by ligating the anterior descending branch of left coronary artery. Then the rats in paeonol group were peritoneally injected paeonol (16.0mg/kg) for 4 weeks;the rats in captopril group were lavaged with captopril (10.0mg/kg) for 4 weeks. The LVWI and collagen volume fraction (CVF) of rats in each group were detected;Immunohistochemical stain was used to detect the expression of TGF-β1 in myocardial tissue. Results:Compared with rats in model group, the LVWI, CVF and TGF-β1 expression in myocardial tissue of rats in paeonol group and captopril group declined obviously (P<0.01). Conclusions: Down regulating TGF-β1 expression in myocardial tissue maybe the mechanisms of paeonol improving ventricular remodeling.