CAJ | 학술논문

目的：：探讨TRIM28基因对肺鳞癌细胞系SK-MES-1细胞生长的影响。方法：采用RNA干扰技术抑制肺鳞癌SK-MES-1细胞TRIM28基因的表达，通过RT-PCR和Western-blot技术检测抑制效果，应用克隆形成实验和MTT实验观察TRIM28基因被抑制后SK-MES-1细胞的增殖和活性。结果：RNA干扰技术能有效抑制TRIM28基因在肺鳞癌SK-MES-1细胞中的表达。TRIM28基因被抑制后，肺鳞癌SK-MES-1细胞的活性和生长受了到明显抑制。结论：TRIM28对肺鳞癌SK-MES-1细胞的生长具有促进作用。
목적：：탐토TRIM28기인대폐린암세포계SK-MES-1세포생장적영향。방법：채용RNA간우기술억제폐린암SK-MES-1세포TRIM28기인적표체，통과RT-PCR화Western-blot기술검측억제효과，응용극륭형성실험화MTT실험관찰TRIM28기인피억제후SK-MES-1세포적증식화활성。결과：RNA간우기술능유효억제TRIM28기인재폐린암SK-MES-1세포중적표체。TRIM28기인피억제후，폐린암SK-MES-1세포적활성화생장수료도명현억제。결론：TRIM28대폐린암SK-MES-1세포적생장구유촉진작용。
Objective:To study the influences of TRIM28 gene on growth of lung squamous cell carcinoma SKMES- 1 cells.Methods:TRIM28 gene was inhibited by RNA interference assay, RT-PCR and Western-blot was used to detect the inhibitory effects. After the TRIM28 gene was inhibited, the proliferation and activity of SK-MES-1 cells were detected respectively by clone formation assay and MTT cell proliferation assay.Results:Expression of TRIM28 in SK-MES-1 cells was inhibited significantly by RNA interference. The activity and growth of SK-MES-1 cells decreased obviously after the TRIM28 gene was inhibited.Conclusions:TRIM28 can promote the growth of lung squamous cell carcinoma SK-MES-1 cells.