井冈山大学学报（自然科学版）
井岡山大學學報（自然科學版）
정강산대학학보（자연과학판）
JOURNAL OF JINGGANGSHAN UNIVERSITY(SCIENCE AND TECHNOLOGY)
2015年
1期
91-94
,共4页
巴中产栀子%肝损伤模型%保肝降酶效应%AST%ALT
巴中產梔子%肝損傷模型%保肝降酶效應%AST%ALT
파중산치자%간손상모형%보간강매효응%AST%ALT
Gardeniae fructus from Bazhong%liver damage model%effect of liver protection and enzyme reduction%AST%ALT
目的：研究巴中产栀子对大鼠肝损伤模型保肝降酶药理效应。方法采用大鼠皮下注射四氯化碳(CCl4)制备肝损伤模型、分别灌胃给予栀子水溶性有效部位、70%醇溶性有效部位，速率法测定天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)，观察巴中、湖北、江西和江津产栀子对CCl4导致的大鼠肝损伤模型降低AST和ALT作用。结果四个产地栀子无论是水溶性有效部位，还是70%醇溶性有效部位均具有非常显著的降低AST和ALT作用（与肝损伤模型组Model比较，分别为P ＜0.001、P ＜0.01、P ＜0.02、P ＜0.01）；栀子水溶性有效部位比70%醇溶性有效部位降低AST、ALT的效果更好，但是无显著性差异（P ＞0.05）。巴中产栀子提取物降低AST、ALT效果比江西、湖北、江津产栀子更好（尤其水溶性有效部位），巴中产栀子水溶性有效部位对肝损伤大鼠AST活性降低作用显著优于江津产栀子水溶性有效部位（P ＜0.05），非常显著优于湖北产栀子水溶性有效部位（P ＜0.01），极显著优于江西产栀子水溶性有效部位（P ＜0.001）；巴中产栀子水溶性有效部位降低ALT作用与湖北、江西、江津产栀子比较有进一步降低的趋势，但是无显著的差异（P ＞0.05）；巴中产栀子70%醇溶性有效部位降低 AST、ALT 作用比江西、湖北、江津产栀子更有优势，但是无显著性差异（P＞0.05）。结论根据栀子对大鼠CCl4肝损伤模型保肝降酶效应研究结果证明巴中产栀子的品质更优、疗效更佳。巴中产栀子作为川产道地药材的开发研究值得各级政府和社会各界的广泛关注。
目的：研究巴中產梔子對大鼠肝損傷模型保肝降酶藥理效應。方法採用大鼠皮下註射四氯化碳(CCl4)製備肝損傷模型、分彆灌胃給予梔子水溶性有效部位、70%醇溶性有效部位，速率法測定天鼕氨痠氨基轉移酶(AST)和丙氨痠氨基轉移酶(ALT)，觀察巴中、湖北、江西和江津產梔子對CCl4導緻的大鼠肝損傷模型降低AST和ALT作用。結果四箇產地梔子無論是水溶性有效部位，還是70%醇溶性有效部位均具有非常顯著的降低AST和ALT作用（與肝損傷模型組Model比較，分彆為P ＜0.001、P ＜0.01、P ＜0.02、P ＜0.01）；梔子水溶性有效部位比70%醇溶性有效部位降低AST、ALT的效果更好，但是無顯著性差異（P ＞0.05）。巴中產梔子提取物降低AST、ALT效果比江西、湖北、江津產梔子更好（尤其水溶性有效部位），巴中產梔子水溶性有效部位對肝損傷大鼠AST活性降低作用顯著優于江津產梔子水溶性有效部位（P ＜0.05），非常顯著優于湖北產梔子水溶性有效部位（P ＜0.01），極顯著優于江西產梔子水溶性有效部位（P ＜0.001）；巴中產梔子水溶性有效部位降低ALT作用與湖北、江西、江津產梔子比較有進一步降低的趨勢，但是無顯著的差異（P ＞0.05）；巴中產梔子70%醇溶性有效部位降低 AST、ALT 作用比江西、湖北、江津產梔子更有優勢，但是無顯著性差異（P＞0.05）。結論根據梔子對大鼠CCl4肝損傷模型保肝降酶效應研究結果證明巴中產梔子的品質更優、療效更佳。巴中產梔子作為川產道地藥材的開髮研究值得各級政府和社會各界的廣汎關註。
목적：연구파중산치자대대서간손상모형보간강매약리효응。방법채용대서피하주사사록화탄(CCl4)제비간손상모형、분별관위급여치자수용성유효부위、70%순용성유효부위，속솔법측정천동안산안기전이매(AST)화병안산안기전이매(ALT)，관찰파중、호북、강서화강진산치자대CCl4도치적대서간손상모형강저AST화ALT작용。결과사개산지치자무론시수용성유효부위，환시70%순용성유효부위균구유비상현저적강저AST화ALT작용（여간손상모형조Model비교，분별위P ＜0.001、P ＜0.01、P ＜0.02、P ＜0.01）；치자수용성유효부위비70%순용성유효부위강저AST、ALT적효과경호，단시무현저성차이（P ＞0.05）。파중산치자제취물강저AST、ALT효과비강서、호북、강진산치자경호（우기수용성유효부위），파중산치자수용성유효부위대간손상대서AST활성강저작용현저우우강진산치자수용성유효부위（P ＜0.05），비상현저우우호북산치자수용성유효부위（P ＜0.01），겁현저우우강서산치자수용성유효부위（P ＜0.001）；파중산치자수용성유효부위강저ALT작용여호북、강서、강진산치자비교유진일보강저적추세，단시무현저적차이（P ＞0.05）；파중산치자70%순용성유효부위강저 AST、ALT 작용비강서、호북、강진산치자경유우세，단시무현저성차이（P＞0.05）。결론근거치자대대서CCl4간손상모형보간강매효응연구결과증명파중산치자적품질경우、료효경가。파중산치자작위천산도지약재적개발연구치득각급정부화사회각계적엄범관주。
Objective: To explore the pharmacological effect of liver protection and enzyme reduction of gardeniae fructus from Bazhong in rat liver damage model. Methods: Preparing rat liver injury model by subcutaneous injection of CCl4, administrating with water extraction and 70% ethanol extraction of gardeniae fructus by intragastric, respectively, determing the activity of AST and ALT by rate method, the reduced activity of AST and ALT on rats with damage liver by CCl4 of gardenia fructus from Bazhong, Hubei, Jiangxi and Jiangjin were investigated. Results: Water or 70% alcohol extraction of gardenia from Bazhong, Hubei, Jiangxi and Jiangjin could significantly reduce the activity of AST and ALT (comparing with model group, P < 0.001, P <0.01, P < 0.02, P < 0.01, respectively); Water extraction was better than 70% ethanol extraction, but no significant difference (P>0.05). The effect of extraction in gardenia from Bazhong that reduced activity of AST and ALT was significantly better than those from Jiangxi, Hubei, and Jiangjin (special for water extraction). The effect of water extraction in gardenia from Bazhong that reduced activity of AST was significantly better than that from Jiangjin (P < 0.05), highly significantly better than that from Hubei (P < 0.01), extremely better than that from Jiangxi (P < 0.001). The effect of water extraction in gardenia from Bazhong that reduced activity of ALT was better than that from Jiangjin, Hubei Jiangxi, but no significant difference (P > 0.05). The effect of 70%ethanol extraction in gardenia from Bazhong that reduced activity of AST and ALT was better than that from Jiangjin, Hubei Jiangxi, but no significant difference (P > 0.05). Conclusion:Quality of gardeniae fructus from Bazhong is better than from Jiangxi, Hubei, and Jiangjin, gardeniae fructus from Bazhong is well worth focusing by government and society.
