临床儿科杂志
臨床兒科雜誌
림상인과잡지
2015年
1期
60-63
,共4页
细胞因子信号转导抑制蛋白%单个核细胞%过敏性紫癜%儿童
細胞因子信號轉導抑製蛋白%單箇覈細胞%過敏性紫癜%兒童
세포인자신호전도억제단백%단개핵세포%과민성자전%인동
suppressors of cytokine signaling%mononuclear cell%Henoch-Sch?enlein purpura%child
目的：探讨细胞因子信号转导抑制蛋白1(SOCS1)和SOCS3在儿童过敏性紫癜中的表达情况和作用。方法应用逆转录—聚合酶链反应检测20例过敏性紫癜患儿和15例正常健康对照儿童的外周血单个核细胞中SOCS1/SOCS3 mRNA表达水平。结果过敏性紫癜患儿SOCS1、SOCS3 mRNA相对表达水平分别为(1.37±0.38)、(1.87±0.53)，高于对照儿童的(0.85±0.12)、(0.77±0.13)，差异均有统计学意义(P均<0.001)；SOCS3 mRNA表达增高更为明显。结论过敏性紫癜患儿有SOCS3的高表达。
目的：探討細胞因子信號轉導抑製蛋白1(SOCS1)和SOCS3在兒童過敏性紫癜中的錶達情況和作用。方法應用逆轉錄—聚閤酶鏈反應檢測20例過敏性紫癜患兒和15例正常健康對照兒童的外週血單箇覈細胞中SOCS1/SOCS3 mRNA錶達水平。結果過敏性紫癜患兒SOCS1、SOCS3 mRNA相對錶達水平分彆為(1.37±0.38)、(1.87±0.53)，高于對照兒童的(0.85±0.12)、(0.77±0.13)，差異均有統計學意義(P均<0.001)；SOCS3 mRNA錶達增高更為明顯。結論過敏性紫癜患兒有SOCS3的高錶達。
목적：탐토세포인자신호전도억제단백1(SOCS1)화SOCS3재인동과민성자전중적표체정황화작용。방법응용역전록—취합매련반응검측20례과민성자전환인화15례정상건강대조인동적외주혈단개핵세포중SOCS1/SOCS3 mRNA표체수평。결과과민성자전환인SOCS1、SOCS3 mRNA상대표체수평분별위(1.37±0.38)、(1.87±0.53)，고우대조인동적(0.85±0.12)、(0.77±0.13)，차이균유통계학의의(P균<0.001)；SOCS3 mRNA표체증고경위명현。결론과민성자전환인유SOCS3적고표체。
Objective To explore the expression and role of suppressor of cytokine signaling 1 (SOCS1) and SOCS3 mRNA in the development of Henoch-Sch?enlein purpura (HSP) in children. Methods Real-Time reverse transcription poly-merase chain reaction (RT-PCR) was used to detect the expression levels of SOCS1 and SOCS3 mRNA in peripheral blood mononuclear cells (PBMCs) in children with HSP (HSP group, n=20) and healthy children (control group, n=15), respectively. Results The expression levels of SOCS1 and SOCS3 mRNA in HSP group were (1.37±0.38) and (1.87±0.53), and in control group were (0.85±0.12) and (0.77±0.13). Compared with control group, the expressions of SOCS1 and SOCS3 mRNA, especially the latter, were signiifcantly up-regulated in HSP group (P<0.001). Conclusions The up-regulated expression of SOCS3 may be involved in the pathogenesis of HSP in children.
