环境保护与循环经济
環境保護與循環經濟
배경보호여순배경제
ENVIRONMENTAL PROTECTION AND CIRCULAR ECONOMY
2015年
1期
65-66
,共2页
酚类化合物%高效液相色谱%固相萃取
酚類化閤物%高效液相色譜%固相萃取
분류화합물%고효액상색보%고상췌취
phenol compounds%high performance liquid chromatography%solid phase extraction(SPE)
建立了一种固相萃取—高效液相色谱法，测定水中的酚类化合物，特别是氯酚和硝基酚几种酚类化合物。吸附前使用4 mL甲醇和4 mL纯水活化固相萃取小柱（SPE），样品通过SPE时酚类化合物被吸附，后用乙腈洗脱，收集洗脱液浓缩至1 mL。以V 水∶V 乙腈为8∶2梯度淋洗，V 水∶V 乙腈为2∶8结束，流速1.0 mL/min，以ODS-C18分离酚类化合物，检测器波长为285 nm。实验结果表明，标准曲线线性关系均大于0.9990；检出限均小于《生活饮用水卫生标准》的要求；平均加标回收率在70%～90%之间。
建立瞭一種固相萃取—高效液相色譜法，測定水中的酚類化閤物，特彆是氯酚和硝基酚幾種酚類化閤物。吸附前使用4 mL甲醇和4 mL純水活化固相萃取小柱（SPE），樣品通過SPE時酚類化閤物被吸附，後用乙腈洗脫，收集洗脫液濃縮至1 mL。以V 水∶V 乙腈為8∶2梯度淋洗，V 水∶V 乙腈為2∶8結束，流速1.0 mL/min，以ODS-C18分離酚類化閤物，檢測器波長為285 nm。實驗結果錶明，標準麯線線性關繫均大于0.9990；檢齣限均小于《生活飲用水衛生標準》的要求；平均加標迴收率在70%～90%之間。
건립료일충고상췌취—고효액상색보법，측정수중적분류화합물，특별시록분화초기분궤충분류화합물。흡부전사용4 mL갑순화4 mL순수활화고상췌취소주（SPE），양품통과SPE시분류화합물피흡부，후용을정세탈，수집세탈액농축지1 mL。이V 수∶V 을정위8∶2제도림세，V 수∶V 을정위2∶8결속，류속1.0 mL/min，이ODS-C18분리분류화합물，검측기파장위285 nm。실험결과표명，표준곡선선성관계균대우0.9990；검출한균소우《생활음용수위생표준》적요구；평균가표회수솔재70%～90%지간。
Phenol compounds especially chlorophenol and nitrophenol compounds in water were determined by SPE-HPLC method. The solid phase extraction (SPE)column was firstly activated by 4 mL methanol and 4 mL pure water, phenol compounds in the sample was absorbed by SPE column, eluted by acetonitrile, and then concentrated to 1 mL. The condition of the HPLC was as follows:pure water and acetonitrile was used as mobile phase and gradient eluted from 8:2 to 2:8, the velocity of flow was 1.0 mL/min. ODS-C18 was used to separate the phenol compounds, the wavelength of the VWD was 285 nm. The linearity of the standard curve was higher than 0.999 0, the detection limits of the phenol compounds was below the requiring of the standard for drinking water quality, the average spike recovery was 70%~90%.
