CAJ | 학술논문

目的研究microRNA-27b在糖尿病大鼠心肌组织中的表达变化和意义。方法建立糖尿病大鼠模型， VG染色观察心肌组织形态及胶原容量，microRNA芯片筛选心肌纤维化相关microRNA，realtime PCR验证microRNA的表达改变。结果 VG染色显示糖尿病组大鼠心肌组织内胶原容量高于对照组（P＜0.05），microRNA芯片提示糖尿病组大鼠与对照组相比心肌组织中多个microRNA出现表达差异，Realtime PCR证实糖尿病大鼠心肌组织中microRNA-27b的表达较对照组上调（P＜0.05）。结论 microRNA-27b可能与糖尿病大鼠心肌纤维化的发生相关。
목적연구microRNA-27b재당뇨병대서심기조직중적표체변화화의의。방법건립당뇨병대서모형， VG염색관찰심기조직형태급효원용량，microRNA심편사선심기섬유화상관microRNA，realtime PCR험증microRNA적표체개변。결과 VG염색현시당뇨병조대서심기조직내효원용량고우대조조（P＜0.05），microRNA심편제시당뇨병조대서여대조조상비심기조직중다개microRNA출현표체차이，Realtime PCR증실당뇨병대서심기조직중microRNA-27b적표체교대조조상조（P＜0.05）。결론 microRNA-27b가능여당뇨병대서심기섬유화적발생상관。
Objective To investigate the expression changes of microRNA- 27b in the cardiac muscle of diabetic rats and its significance. Methods After establishment of diabetic rat model, van Gieson stain was used to determine myocardial morphology and collagen volume, microRNA chips were used to screen the microRNAs associated with myocardial fibrosis, and realtime PCR was performed to validate the expression changes of microRNA. Results Van Gieson stain displayed that the collagen volume of cardiac muscle in the diabetic rats was higher than that in control group (P <0.05). MicroRNA chips uncovered abnormal expression of several microRNAs and realtime PCR validated up- regulation of microRNA- 27b expression in cardiac muscle of diabetic rats compared with control group (P<0.05). Conclusion MicroRNA- 27b may be associated with myocardial fibrosis of diabetic rats.