CAJ | 학술논문

目的：通过动物试验探讨透骨消痛颗粒对骨性关节炎早期环氧化酶-2（COX-2）和诱导性一氧化氮合酶（iNOS）表达的影响，研究透骨消痛颗粒在膝骨性关节炎治疗中的相关作用机制．方法：新西兰大白兔采用改良 Hulth 法造模，造模成功后随机分成3组：模型组、对照组、实验组，和正常组共4组，分别灌胃给药4周后处死动物取材，并观测如下指标：关节腔大体观察，滑膜与软骨组织学观察；关节液内 NO、NOS、PGE2含量检测，滑膜软骨中 iNOS、COX-2含量检测．结果：4周后关节液中 NO、NOS 含量、PGE2浓度，滑膜和软骨中iNOS、COX-2含量，模型组、对照组、实验组均呈高表达，与正常组比较差异有显著性（P ＜0．05）；对照组、实验组与模型组比较差异有显著性（P ＜0．05）；对照组与实验组比较差异有显著性（P ＜0．05）．结论：透骨消痛颗粒可通过抑制 COX-2和 iNOS 在 OA 早期滑膜和软骨细胞中的表达及其诱导产物PGE2和 NO 的生成，从而达到控制 OA 早期炎症、减轻疼痛、缓解症状的作用．
목적：통과동물시험탐토투골소통과립대골성관절염조기배양화매-2（COX-2）화유도성일양화담합매（iNOS）표체적영향，연구투골소통과립재슬골성관절염치료중적상관작용궤제．방법：신서란대백토채용개량 Hulth 법조모，조모성공후수궤분성3조：모형조、대조조、실험조，화정상조공4조，분별관위급약4주후처사동물취재，병관측여하지표：관절강대체관찰，활막여연골조직학관찰；관절액내 NO、NOS、PGE2함량검측，활막연골중 iNOS、COX-2함량검측．결과：4주후관절액중 NO、NOS 함량、PGE2농도，활막화연골중iNOS、COX-2함량，모형조、대조조、실험조균정고표체，여정상조비교차이유현저성（P ＜0．05）；대조조、실험조여모형조비교차이유현저성（P ＜0．05）；대조조여실험조비교차이유현저성（P ＜0．05）．결론：투골소통과립가통과억제 COX-2화 iNOS 재 OA 조기활막화연골세포중적표체급기유도산물PGE2화 NO 적생성，종이체도공제 OA 조기염증、감경동통、완해증상적작용．
AIM:To investgate the mechanism of influence a-bout granula of penetrating bone removeing pain intervene the ex-pression of COX-2 and iNOS in early stage of osteoarthritis by ani-mals experiment,to investgate mechanism of granula of penetrat-ing bone removeing pain on treatment of osteoarthritis.METH-ODS:We devided the New Zealand rabbits into 3 groups random-ly after replicated knee osteoarthritis with modified Hulth modeling method:model group,control group,experimental group,with the normal group were 4 groups in total.We execute all rabbits af-ter they were fed intragastically for 4 weekes.Then the following detection were taken out:General Observation of articular cavity;histological Conversation of synovium and cartilage;content of NOS,NO,test of iNOSmRNA,concentration of COX-2 and PGE2 within joint.RESULTS:After four weekes,there was sig-nificant contrast (P <0.05 )of the concent of NOS,NO,iN-OSmRNA,COX-2 and PGE2 of model group,control group,ex-perimental group compared with the normal group;There was sig-nificant contrast (P <0.05 )of the concent of NOS,NO,iN-OSmRNA,COX-2 and PGE2 of experimental groups and control group compared with the model group;There was significant con-trast (P <0.05)of the concent of NOS,NO,iNOSmRNA,COX-2 and PGE2 of experimental group compared with control group. CONCLUSION:The granula of penetrating bone removeing pain can control inflammation,relieve pains and alleviate symptoms in early stage of osteoarthritis by inhibiting the expression of COX-2 and iNOS within cartilage and synovium and releving the produc-tion of PGE2 and NO which can be inducktived by COX-2 and iN-OS.