CAJ | 학술논문

目的：探讨沉默缺氧诱导因子-lα（hypoxia inducible factor-lα，HIF-lα）基因对前列腺癌PC3细胞生长增殖及细胞凋亡的影响。方法实验分为PC3组（空白对照组）、PC3+NC siRNA组（阴性对照组）和PC3+HIF-lαsiRNA组（干扰组）。使用经过筛选证实有效的HIF-lα-siRNA序列和阴性对照NC-siRNA序列，经脂质体Lipofectamine 2000转染PC3细胞。采用CCK-8法检测转染后96h内各组细胞的生长增殖情况，流式细胞仪检测转染后48h各组细胞的凋亡率。结果 PC3+HIF-lαsiRNA组肿瘤抑制率高于PC3+NC siRNA组，尤以转染后第48h表现得较为明显；在第48h，PC3+HIF-lαsiRNA组细胞存活率明显低于PC3组和PC3+NCsiRNA组（P＜0.01）。转染后第48h，干扰组细胞凋亡率为（11.2±1.13）%，PC3组和PC3+NC siRNA组细胞凋亡率分别为（2.4±0.26）%和（2.5±0.36）%，干扰组细胞凋亡率显著高于两对照组（P＜0.01）。结论沉默HIF-lα基因能抑制前列腺癌PC3细胞的生长，诱导癌细胞凋亡，发挥抗肿瘤作用。
목적：탐토침묵결양유도인자-lα（hypoxia inducible factor-lα，HIF-lα）기인대전렬선암PC3세포생장증식급세포조망적영향。방법실험분위PC3조（공백대조조）、PC3+NC siRNA조（음성대조조）화PC3+HIF-lαsiRNA조（간우조）。사용경과사선증실유효적HIF-lα-siRNA서렬화음성대조NC-siRNA서렬，경지질체Lipofectamine 2000전염PC3세포。채용CCK-8법검측전염후96h내각조세포적생장증식정황，류식세포의검측전염후48h각조세포적조망솔。결과 PC3+HIF-lαsiRNA조종류억제솔고우PC3+NC siRNA조，우이전염후제48h표현득교위명현；재제48h，PC3+HIF-lαsiRNA조세포존활솔명현저우PC3조화PC3+NCsiRNA조（P＜0.01）。전염후제48h，간우조세포조망솔위（11.2±1.13）%，PC3조화PC3+NC siRNA조세포조망솔분별위（2.4±0.26）%화（2.5±0.36）%，간우조세포조망솔현저고우량대조조（P＜0.01）。결론침묵HIF-lα기인능억제전렬선암PC3세포적생장，유도암세포조망，발휘항종류작용。
Objective To investigate the effects of hypoxia inducible factor-lα gene silencing with siRNA on the proliferation and apoptosis of human prostate cancer cells. Methods PC3 cells were divided into three groups such as PC3 group, PC3+ NC siRNA group and PC3 + HIF-lα siRNA group. Cells in each group were transfected with HIF-lα-siRNA using Lipofectamine 2000. CCK-8 test was used to measure the proliferation of PC3 cells. Flow cytometer was used to measure cell apoptosis. Results Survival rate of PC3+HIF-lα siRNA group was significantly lower than that of other two control groups at 24h, 48h, 72h and 96h after transfection (P<0.01). The interference effect reached its peak at 48h after transfection. The rates of cell apoptosis of PC3 + HIF-lα siRNA group, PC3 group, PC3+ NC siRNA group at 48h after transfection were (11.2±1.13)%,(2.4±0.26)% and (2.5±0.36)% respectively; The apoptosis rates of PC3 + HIF-lαsiRNA group was higher than that of the others (P<0.01). Conclusion Silencing HIF-lα gene can effectively inhibit cell proliferation, and induce the apoptosis of PC3 cells.