CAJ | 학술논문

研究多西环素对甲状腺癌SW579细胞增殖、凋亡和侵袭的影响。采用MTT技术检测细胞增殖能力的变化，荧光分光光度计检测线粒体膜电位的变化，Westernblot技术检测细胞色素C（CytC）和基质金属蛋白酶2（MMP‐2）蛋白表达，Transwell小室侵袭实验检测细胞侵袭能力的变化。结果显示，与对照组相比，多西环素组细胞存活率，线粒体膜电位，MMP‐2蛋白表达，侵袭细胞数均明显降低（P＜0．05），CytC表达明显增高（P＜0．05），呈浓度和时间依赖性（P＜0．05）。因此，多西环素能有效抑制SW579细胞增殖和侵袭，并且能诱导其细胞凋亡。
연구다서배소대갑상선암SW579세포증식、조망화침습적영향。채용MTT기술검측세포증식능력적변화，형광분광광도계검측선립체막전위적변화，Westernblot기술검측세포색소C（CytC）화기질금속단백매2（MMP‐2）단백표체，Transwell소실침습실험검측세포침습능력적변화。결과현시，여대조조상비，다서배소조세포존활솔，선립체막전위，MMP‐2단백표체，침습세포수균명현강저（P＜0．05），CytC표체명현증고（P＜0．05），정농도화시간의뢰성（P＜0．05）。인차，다서배소능유효억제SW579세포증식화침습，병차능유도기세포조망。
To detect the effects of doxycycline on cell proliferation ,apoptosis ,and invasion as well as the metastasis of of thyroid squamous cell carcinoma cell line SW579 .The ability of cell proliferation in each group was detected using MTT assay ,the mitochondrial membrane potential was measured with fluorescence spectrophotometer ,the expression levels of cytochrome C (CytC) and MMP‐2 in each group were measured by western blot ,the ability of invasion in each group was measured with Transwell assays .The results showed that the cell survival rates ,invasion rate and the MMP‐2 expression of SW579 decreased dramatically (P<0 .05) ,while the expression of CytC increased significantly in the doxycycline group compared with control group (P< 0 .05) ,which are all in a concentration and time‐dependent manners (P< 0 .05) . Doxycycline can effectively inhibit the cell proliferation and invasion ,and induce apoptosis of SW579 cells .