中国循环杂志
中國循環雜誌
중국순배잡지
CHINESE CIRCULATION JOURNAL
2015年
2期
154-158
,共5页
王晓建%黄毕%樊晓寒%苏文君%张良%路天怡%田力%杨艳敏%惠汝太%张澍
王曉建%黃畢%樊曉寒%囌文君%張良%路天怡%田力%楊豔敏%惠汝太%張澍
왕효건%황필%번효한%소문군%장량%로천이%전력%양염민%혜여태%장주
miRNA芯片%急性主动脉夹层%miRNA差异表达
miRNA芯片%急性主動脈夾層%miRNA差異錶達
miRNA심편%급성주동맥협층%miRNA차이표체
miRNA chips%Acute aortic dissection%miRNA differential expression
目的：本研究旨在寻找新的可作为急性主动脉夹层（AAD）诊断标志物的微小核糖核酸(miRNAs)。
<br> 方法：入选4例A型AAD患者的升主动脉病变组织标本（AAD1组）和4例无心血管疾病的器官捐赠者的升主动脉组织标本（NC1组）；入选20例A型AAD患者（AAD2组）和20例无心血管疾病正常对照者（NC2组）并分别采集血浆，两组病例对照的年龄、性别严格匹配。利用miRNA芯片技术，检测四组受试者的升主动脉组织和血浆miRNAs表达谱，整合两组分析结果，确定AAD患者组织和血浆均差异表达的miRNAs。
<br> 结果：主动脉组织miRNA芯片结果显示，AAD患者中30个miRNAs表达显著改变，其中13个表达上调，17个表达下调。血浆miRNA芯片分析显示，AAD患者有93个miRNA差异表达，其中33个表达上调，60个表达下调。整合两个表达谱，共有4个miRNAs（miR-4313、-933、-1281和-1238）在血浆和组织均显著升高。其中，AAD1组较NC1组、AAD2组较NC2组miR-4313分别上调1.5倍和42.4倍，miR-933分别上调10.4倍和26.8倍，miR-1281分别上调1.7倍和17.8倍，miR-1238分别上调1.3倍和13.8倍。
<br> 结论：通过miRNA芯片分析，我们筛选到4个在AAD患者主动脉组织和血浆中均升高的miRNAs。这些miRNA可能是诊断AAD的生物标志物，但需大样本验证。
目的：本研究旨在尋找新的可作為急性主動脈夾層（AAD）診斷標誌物的微小覈糖覈痠(miRNAs)。
<br> 方法：入選4例A型AAD患者的升主動脈病變組織標本（AAD1組）和4例無心血管疾病的器官捐贈者的升主動脈組織標本（NC1組）；入選20例A型AAD患者（AAD2組）和20例無心血管疾病正常對照者（NC2組）併分彆採集血漿，兩組病例對照的年齡、性彆嚴格匹配。利用miRNA芯片技術，檢測四組受試者的升主動脈組織和血漿miRNAs錶達譜，整閤兩組分析結果，確定AAD患者組織和血漿均差異錶達的miRNAs。
<br> 結果：主動脈組織miRNA芯片結果顯示，AAD患者中30箇miRNAs錶達顯著改變，其中13箇錶達上調，17箇錶達下調。血漿miRNA芯片分析顯示，AAD患者有93箇miRNA差異錶達，其中33箇錶達上調，60箇錶達下調。整閤兩箇錶達譜，共有4箇miRNAs（miR-4313、-933、-1281和-1238）在血漿和組織均顯著升高。其中，AAD1組較NC1組、AAD2組較NC2組miR-4313分彆上調1.5倍和42.4倍，miR-933分彆上調10.4倍和26.8倍，miR-1281分彆上調1.7倍和17.8倍，miR-1238分彆上調1.3倍和13.8倍。
<br> 結論：通過miRNA芯片分析，我們篩選到4箇在AAD患者主動脈組織和血漿中均升高的miRNAs。這些miRNA可能是診斷AAD的生物標誌物，但需大樣本驗證。
목적：본연구지재심조신적가작위급성주동맥협층（AAD）진단표지물적미소핵당핵산(miRNAs)。
<br> 방법：입선4례A형AAD환자적승주동맥병변조직표본（AAD1조）화4례무심혈관질병적기관연증자적승주동맥조직표본（NC1조）；입선20례A형AAD환자（AAD2조）화20례무심혈관질병정상대조자（NC2조）병분별채집혈장，량조병례대조적년령、성별엄격필배。이용miRNA심편기술，검측사조수시자적승주동맥조직화혈장miRNAs표체보，정합량조분석결과，학정AAD환자조직화혈장균차이표체적miRNAs。
<br> 결과：주동맥조직miRNA심편결과현시，AAD환자중30개miRNAs표체현저개변，기중13개표체상조，17개표체하조。혈장miRNA심편분석현시，AAD환자유93개miRNA차이표체，기중33개표체상조，60개표체하조。정합량개표체보，공유4개miRNAs（miR-4313、-933、-1281화-1238）재혈장화조직균현저승고。기중，AAD1조교NC1조、AAD2조교NC2조miR-4313분별상조1.5배화42.4배，miR-933분별상조10.4배화26.8배，miR-1281분별상조1.7배화17.8배，miR-1238분별상조1.3배화13.8배。
<br> 결론：통과miRNA심편분석，아문사선도4개재AAD환자주동맥조직화혈장중균승고적miRNAs。저사miRNA가능시진단AAD적생물표지물，단수대양본험증。
Objective: We want to identify the new microRNAs (miRNAs) biomarker for diagnosing the patients with acute aortic dissection (AAD).
<br> Methods: Our work included 2 sets of groups.①AAD 1 tissue group, containing the aortic tissue of A type AAD patients and Control 1 group, containing the aortic tissue of the subjects without cardiovascular disease. n=4 in each group. ② AAD 2 plasma group, containing the plasma of A type AAD patients and Control 2 group, containing the plasma of the subjects without cardiovascular disease. n=20 in each group. The age and gender were strictly matched between the patients and control subjects. The miRNAs expression in ascending aortic tissue and plasma were examined by microarray analysis in 4 groups, and the results in 2 sets of groups were integrated to identify the differential expression of miRNAs in AAD patients.
<br> Results: The microarray analysis indicated that in AAD patients, the aortic tissue had 30 differentially expressed
<br> miRNAs, 13 of them were up-regulated and 17 were down-regulated;plasma had 93 differentially expressed miRNAs, 33 of them were up-regulated and 60 were down-regulated. With integrated analysis, 4 miRNAs expressions were increased in both aortic tissue and plasma as miR-4313, miR-933, miR-1281 and miR-1238. Compared with Control 1 group and Control 2 group, AAD 1 group and AAD 2 group showed up-regulated miR-4313 at 1.5-fold and 42.4-fold, up-regulated miR-933 at 10.4-fold and 26.8-fold, up-regulated miR-1281 at 1.7-fold and 17.8-fold, up-regulated miR-1238 at 1.3-fold and 13.8-fold respectively.
<br> Conclusion: There were 4 differentially expressed miRNAs in both aortic tissue and plasma in AAD patients, which might be the potential biomarkers of AAD, while large sample investigation is needed.
