中国中西医结合肾病杂志
中國中西醫結閤腎病雜誌
중국중서의결합신병잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN NEPHROLOGY
2015年
2期
113-115
,共3页
雷公藤多苷%糖尿病肾病%甘露糖结合凝集素%白细胞介素6%肿瘤坏死因子α
雷公籐多苷%糖尿病腎病%甘露糖結閤凝集素%白細胞介素6%腫瘤壞死因子α
뢰공등다감%당뇨병신병%감로당결합응집소%백세포개소6%종류배사인자α
Triptergium wilfordii polyglucosides%Diabetic nephropathy%Mannan-binding lectin%Interleukin 6%Tis-sue necrosis factor-α
目的：：观察雷公藤多苷( multiglucosides of triptergium wilfordii,GTW)对糖尿病肾病( diabetic nephropathy,DN)大鼠肾组织甘露糖结合凝集素(mannose-binding lectin,MBL)、白细胞介素6(interleukin 6,IL-6)、肿瘤坏死因子α(tissue necrosis factor-α,TNF-α)表达影响,探讨MBL、IL-6、TNF-α与DN关系,阐明GTW对DN保护作用及可能机制。方法：40只雄性SD大鼠随机分为实验组和对照组,实验组高糖高脂饮食6周后,以链脲佐菌素( streptozocin,STZ)建立DN大鼠模型。造模成功的大鼠随机分为DN组和GTW治疗组,治疗组经口灌胃GTW(10 mg·kg-1·d-1)8周。第14周末称体重并查24 h尿蛋白定量、血清生化指标、观察肾脏结构及炎细胞浸润程度,免疫组化法检测MBL-A、IL-6、TNF-α蛋白在肾脏中的表达,RT-PCR法检测肾组织MBL1mRNA表达情况。结果：GTW可减轻DN大鼠蛋白尿,降低血清肌酐、尿素氮水平,下调肾组织MBL、IL-6、TNF-α表达。结论：GTW对DN大鼠肾组织有保护作用,可能与GTW减少了DN大鼠肾组织MBL、IL-6、TNF-α表达,减轻了免疫炎症反应对肾脏的损伤有关。
目的：：觀察雷公籐多苷( multiglucosides of triptergium wilfordii,GTW)對糖尿病腎病( diabetic nephropathy,DN)大鼠腎組織甘露糖結閤凝集素(mannose-binding lectin,MBL)、白細胞介素6(interleukin 6,IL-6)、腫瘤壞死因子α(tissue necrosis factor-α,TNF-α)錶達影響,探討MBL、IL-6、TNF-α與DN關繫,闡明GTW對DN保護作用及可能機製。方法：40隻雄性SD大鼠隨機分為實驗組和對照組,實驗組高糖高脂飲食6週後,以鏈脲佐菌素( streptozocin,STZ)建立DN大鼠模型。造模成功的大鼠隨機分為DN組和GTW治療組,治療組經口灌胃GTW(10 mg·kg-1·d-1)8週。第14週末稱體重併查24 h尿蛋白定量、血清生化指標、觀察腎髒結構及炎細胞浸潤程度,免疫組化法檢測MBL-A、IL-6、TNF-α蛋白在腎髒中的錶達,RT-PCR法檢測腎組織MBL1mRNA錶達情況。結果：GTW可減輕DN大鼠蛋白尿,降低血清肌酐、尿素氮水平,下調腎組織MBL、IL-6、TNF-α錶達。結論：GTW對DN大鼠腎組織有保護作用,可能與GTW減少瞭DN大鼠腎組織MBL、IL-6、TNF-α錶達,減輕瞭免疫炎癥反應對腎髒的損傷有關。
목적：：관찰뢰공등다감( multiglucosides of triptergium wilfordii,GTW)대당뇨병신병( diabetic nephropathy,DN)대서신조직감로당결합응집소(mannose-binding lectin,MBL)、백세포개소6(interleukin 6,IL-6)、종류배사인자α(tissue necrosis factor-α,TNF-α)표체영향,탐토MBL、IL-6、TNF-α여DN관계,천명GTW대DN보호작용급가능궤제。방법：40지웅성SD대서수궤분위실험조화대조조,실험조고당고지음식6주후,이련뇨좌균소( streptozocin,STZ)건립DN대서모형。조모성공적대서수궤분위DN조화GTW치료조,치료조경구관위GTW(10 mg·kg-1·d-1)8주。제14주말칭체중병사24 h뇨단백정량、혈청생화지표、관찰신장결구급염세포침윤정도,면역조화법검측MBL-A、IL-6、TNF-α단백재신장중적표체,RT-PCR법검측신조직MBL1mRNA표체정황。결과：GTW가감경DN대서단백뇨,강저혈청기항、뇨소담수평,하조신조직MBL、IL-6、TNF-α표체。결론：GTW대DN대서신조직유보호작용,가능여GTW감소료DN대서신조직MBL、IL-6、TNF-α표체,감경료면역염증반응대신장적손상유관。
Objective:To observe the effects of multiglucosides of triptergium wilfordii on the expression of Man-nose-Binding Lectin(MBL)、interleukin 6(IL-6)、tissue necrosis factor-α(TNF-α) in renal tissue of diabetic nephropathy rats, dis-cussing the relationship between MBL、IL-6、TNF-α and diabetic nephropathy. In order to explore the potential protective mecha-nism of multiglucosides of triptergium wilfordii on diabetic nephropathy. Methods:Forty male Sprague-Dawlay( SD) rats were ran-domly divided into experimental group and normal control group, after 6 weeks high-suger and high-fat diet, the experimental group diabetes were injected into streptozocin( STZ) for DN rat model. The Rat models were randomly divided into diabetes mellitus control group ( group DN) , and multiglucosides of triptergium wilfordii treated diabetes mellitus group( group GTW) , that were treated with multiglucosides of triptergium wilfordii (10 mg·kg-1 ·d-1 ) for 8 weeks. After 14 weeks, the weight of rat、24 hours protein(24 h U-TP)、biochemical indexes were measured. Partial nephridial tissues were treated with HE staining. Using immunohistochemistry to observe the protein expression of MBL-A、IL-6、TNF-αand RT-PCR to observe the expression of MBL1 mRNA. Results:Com-pared with the untreated diabetic group, urinary protein excretion, serum creatinine、serum urea nitrogen of GTW treated rats were substanitially decreased. The level of MBL-A、IL-6、TNF-α increased in DN group and then decreased when treated with GTW. Conclusion:GTW can reduce the injury of immune inflammatory response in DN rats, partly through down-regulating the expression of MBL-A、IL-6 and TNF-α.
