CAJ | 학술논문

目的：：观察参地补肾胶囊对肾小球硬化大鼠基质金属蛋白酶(MMP-9)、基质金属蛋白酶抑制物(TIMP-1)及其受体mRNA表达的影响,探讨其抗肾小球硬化的机制。方法：采用单侧肾脏切除同时尾静脉注射阿霉素的方法建立肾小球硬化大鼠模型,SD大鼠随机分为空白对照组、模型组、参地补肾胶囊组、贝那普利组、苏黄泄浊丸组,检测血肌酐( Scr)、尿素氮( BUN)、血浆蛋白( Alb)；免疫组化法检测MMP-9、TIMP-1的表达,计算MMP-9/TIMP-1比值,RT-PCR检测受体TIMP-1mRNA的表达水平。结果：与模型组比较,参地补肾胶囊组能抑制TIMP-1及其受体mRNA的表达(P<0.01),上调MMP-9表达(P<0.01),提高MMP-9/TIMP-1的比值。结论：参地补肾胶囊可抑制TIMP-1及其受体mRNA的表达,改善肾脏病理形态,进而延缓肾小球硬化进展。
목적：：관찰삼지보신효낭대신소구경화대서기질금속단백매(MMP-9)、기질금속단백매억제물(TIMP-1)급기수체mRNA표체적영향,탐토기항신소구경화적궤제。방법：채용단측신장절제동시미정맥주사아매소적방법건립신소구경화대서모형,SD대서수궤분위공백대조조、모형조、삼지보신효낭조、패나보리조、소황설탁환조,검측혈기항( Scr)、뇨소담( BUN)、혈장단백( Alb)；면역조화법검측MMP-9、TIMP-1적표체,계산MMP-9/TIMP-1비치,RT-PCR검측수체TIMP-1mRNA적표체수평。결과：여모형조비교,삼지보신효낭조능억제TIMP-1급기수체mRNA적표체(P<0.01),상조MMP-9표체(P<0.01),제고MMP-9/TIMP-1적비치。결론：삼지보신효낭가억제TIMP-1급기수체mRNA적표체,개선신장병리형태,진이연완신소구경화진전。
Objective:To observe the effects of shendi bushen capsule on the m RNA expressions of MMP-9, TIMP-1 and its receptor in rats with glomerulosclerosis ( GS) . Methods:GS rat model was established by unilateral nephrectomy and adriamy-cin injection. SD rats were randomly divided into blank control group, model group, shendi bushen capsule group, benazepril group and suhuang xiezhuo pill group. Serum creatinine ( Scr) , blood urea nitrogen ( BUN) and plasma albumin ( Alb) were detected. Im-munohistochemical method was used to detect the expressions of MMP-9 and TIMP-1, and the ratio of MMP-9/TIMP-1 was cal-culated. RT-PCR was used to detect the expression level of TIMP-1 receptor m RNA. Results:Compared with the model group, shendi bushen capsule could obviously inhibit TIMP-1 and its receptor m RNA expressions (P<0. 01), increase MMP-9 expres-sion (P<0. 01), and enhance the ratio of MMP-9/TIMP-1. Conclusion:Shendi bushen capsule can inhibit the expressions of TIMP-1 and its receptor m RNA, improve the renal function so that it may retard the development of glomerular sclerosis.