浙江农业学报
浙江農業學報
절강농업학보
ACTA AGRICULTURAE ZHEJIANGENSIS
2015年
2期
176-181
,共6页
生菜%半致死温度%耐热性%Logistic方程%抗氧化系统
生菜%半緻死溫度%耐熱性%Logistic方程%抗氧化繫統
생채%반치사온도%내열성%Logistic방정%항양화계통
lettuce%semi-lethal high temperature%heat tolerance%Logistic equation%antioxidant system
为探明生菜不同品种对高温的适应性差异,以生菜叶片为试验材料,以半致死温度及抗氧化酶活性为参照指标,对7个生菜品种,A(战神)、B(玉湖)、C(剑客)、D(小叶苦苣)、E(申选5号)、F(玛丽娜)、G(橡叶绿)的耐热性进行研究。结果表明,随着处理温度的升高,7个生菜品种叶片的细胞伤害率增长速度均呈现“慢—快—慢”的趋势,呈典型的“S”型曲线,显著性检验均符合Logistic方程。7个生菜品种耐高温能力由强到弱依次为C(49.67℃)>D(49.60℃)>F(49.36℃)>B(49.13℃)>G(48.81℃)>E(48.74℃)>A (47.86℃)。同时,高温处理6 d后,各品种的超氧化物歧化酶( SOD)、过氧化物酶( POD)、过氧化氢酶( CAT)活性和丙二醛( MDA)含量均比对照有不同程度的上升;半致死温度与MDA含量及SOD、POD、CAT等抗氧化酶活性呈显著相关性,以上结果说明半致死温度可以作为鉴定生菜耐热性强弱的指标。
為探明生菜不同品種對高溫的適應性差異,以生菜葉片為試驗材料,以半緻死溫度及抗氧化酶活性為參照指標,對7箇生菜品種,A(戰神)、B(玉湖)、C(劍客)、D(小葉苦苣)、E(申選5號)、F(瑪麗娜)、G(橡葉綠)的耐熱性進行研究。結果錶明,隨著處理溫度的升高,7箇生菜品種葉片的細胞傷害率增長速度均呈現“慢—快—慢”的趨勢,呈典型的“S”型麯線,顯著性檢驗均符閤Logistic方程。7箇生菜品種耐高溫能力由彊到弱依次為C(49.67℃)>D(49.60℃)>F(49.36℃)>B(49.13℃)>G(48.81℃)>E(48.74℃)>A (47.86℃)。同時,高溫處理6 d後,各品種的超氧化物歧化酶( SOD)、過氧化物酶( POD)、過氧化氫酶( CAT)活性和丙二醛( MDA)含量均比對照有不同程度的上升;半緻死溫度與MDA含量及SOD、POD、CAT等抗氧化酶活性呈顯著相關性,以上結果說明半緻死溫度可以作為鑒定生菜耐熱性彊弱的指標。
위탐명생채불동품충대고온적괄응성차이,이생채협편위시험재료,이반치사온도급항양화매활성위삼조지표,대7개생채품충,A(전신)、B(옥호)、C(검객)、D(소협고거)、E(신선5호)、F(마려나)、G(상협록)적내열성진행연구。결과표명,수착처리온도적승고,7개생채품충협편적세포상해솔증장속도균정현“만—쾌—만”적추세,정전형적“S”형곡선,현저성검험균부합Logistic방정。7개생채품충내고온능력유강도약의차위C(49.67℃)>D(49.60℃)>F(49.36℃)>B(49.13℃)>G(48.81℃)>E(48.74℃)>A (47.86℃)。동시,고온처리6 d후,각품충적초양화물기화매( SOD)、과양화물매( POD)、과양화경매( CAT)활성화병이철( MDA)함량균비대조유불동정도적상승;반치사온도여MDA함량급SOD、POD、CAT등항양화매활성정현저상관성,이상결과설명반치사온도가이작위감정생채내열성강약적지표。
To detect the adaptability difference under high temperature of different lettuce cultivars, leaf segments of seven lettuce cultivars ( A-G) were used to study the heat tolerance by electrical conductivity method and antioxidant enzyme indexes.The results showed that with the rise of treated temperature, cells injured rate of leaf segments from A to G were increased with trends of slow-fast-slow, showed typical S-type curve, the significance test of which was fit Logistic equation.The heat resistance of the seven lettuce cultivars followed the order: C>D>F>B>G>E>A, respectively.Meanwhile, treating with high temperature for 6 d increased the superoxidase ( SOD) , peroxidase ( POD) , catalase ( CAT) activities and the malondisldehyde ( MDA) contents of various lettuce cultivars.The rela-tionship between semi-lethal high temperature and antioxidant enzyme indexes was significant.The above results showed that semi-lethal high temperature could be considered as the index of lettuce heat resistance.