CAJ | 학술논문

收集屠宰场水牛卵巢卵母细胞，在卵子体外成熟和受精后胚胎体外发育过程中分别添加0（对照组），10，50，100和200μmol· L－1的亚麻酸，探究亚麻酸对水牛卵母细胞核成熟率、受精卵的分裂率和植入前胚胎体外发育的影响。结果显示：在水牛卵母细胞体外成熟液中添加亚麻酸，添加浓度50μmol· L－1组的核成熟率（74.18％）显著高于对照组和其他实验组（ P＜0.05），囊胚率（33.24％）显著高于对照组和10μmol· L－1亚麻酸组（P＜0.05）。在胚胎培养液中添加50μmol· L－1亚麻酸组的囊胚率（34.52％）显著高于对照组和100μmol· L－1亚麻酸组（ P＜0.05）；同时在成熟液和胚胎培养液中添加亚麻酸，添加浓度200μmol· L－1组的分裂率显著高于对照组（ P＜0.05），而各组间的囊胚率和D7囊胚比率均差异不显著。结果表明，单独在水牛卵母细胞体外成熟液和胚胎培养液中添加50μmol· L－1亚麻酸能有效促进水牛卵母细胞体外成熟和囊胚发育。
수집도재장수우란소란모세포，재란자체외성숙화수정후배태체외발육과정중분별첨가0（대조조），10，50，100화200μmol· L－1적아마산，탐구아마산대수우란모세포핵성숙솔、수정란적분렬솔화식입전배태체외발육적영향。결과현시：재수우란모세포체외성숙액중첨가아마산，첨가농도50μmol· L－1조적핵성숙솔（74.18％）현저고우대조조화기타실험조（ P＜0.05），낭배솔（33.24％）현저고우대조조화10μmol· L－1아마산조（P＜0.05）。재배태배양액중첨가50μmol· L－1아마산조적낭배솔（34.52％）현저고우대조조화100μmol· L－1아마산조（ P＜0.05）；동시재성숙액화배태배양액중첨가아마산，첨가농도200μmol· L－1조적분렬솔현저고우대조조（ P＜0.05），이각조간적낭배솔화D7낭배비솔균차이불현저。결과표명，단독재수우란모세포체외성숙액화배태배양액중첨가50μmol· L－1아마산능유효촉진수우란모세포체외성숙화낭배발육。
This study was conducted to investigate the effect of different linolenic acid ( ALA) concentrations ( 0, 10, 50, 100 and 200μmol· L-1) during in vitro oocyte maturation (IVM) and in vitro embryo culture (IVC) on the maturation of oocytes and developmental competence of preimplantation embryo in buffalo.The oocytes from abattoir ovaries were matured and fertilized, and presumptive zygotes were cultured in the medium, then the maturation rate cleavage and blastocyst development rate were examined, respectively.The results showed that the maturation rate of oocytes treated with 50 μmol· L-1 ALA was significantly increased ( 74.18%) compared with the control and other supplemental groups ( P<0.05 ) , and the blastocyst development rate of oocytes treated with 50 μmol· L-1 ALA (33.24%) was significantly higher than those of the control and 10 μmol· L-1 ALA supplemental groups ( P <0.05).The presence of 50 μmol· L-1 ALA in IVC resulted in a significantly higher blastocyst development rate (34.52%) compared to the control and 100μmol· L-1 ALA supplemental groups (P<0.05);The ALA with differ-ent concentrations were added to both IVM and IVC, cleavage rates in the group of 200 μmol· L-1 ALA was signifi-cantly higher than that of the control (P<0.05), while blastocyst development rate and percentage of Day 7 blasto-cysts showed no difference.In conclusion, addition of 50 μmol· L-1 ALA to either IVM or IVC in vitro can promote the maturation rate of buffalo oocytes and blastocyst development.