CAJ | 학술논문

目的：探讨脂代谢相关microRNA靶基因结合位点多态性与 T2DM 遗传易感性的关系。方法采用病例‐对照研究，针对生物信息学预测筛选的脂代谢相关microRNA靶基因结合区8个多态性位点（SNPs），应用Taqman‐MGB探针技术，检测全部研究对象（T2DM 患者874例，正常对照者896名）SNP位点的基因型，分析其不同基因型在中国东南方汉族人群中频率的分布及其与 T2DM 的关系。结果校正年龄、性别、WC后，CBY1基因的rs1065201（T＞ C）与 T2DM 的发生呈正相关[OR（95％ CI）为1.51（1.13～2.01）]。结论脂代谢相关microRNA靶基因结合区域多态性与 T2DM 遗传易感性相关，但有待在更多的人群中得到重复验证，以及功能性研究的支持，进一步验证其病因联系。
목적：탐토지대사상관microRNA파기인결합위점다태성여 T2DM 유전역감성적관계。방법채용병례‐대조연구，침대생물신식학예측사선적지대사상관microRNA파기인결합구8개다태성위점（SNPs），응용Taqman‐MGB탐침기술，검측전부연구대상（T2DM 환자874례，정상대조자896명）SNP위점적기인형，분석기불동기인형재중국동남방한족인군중빈솔적분포급기여 T2DM 적관계。결과교정년령、성별、WC후，CBY1기인적rs1065201（T＞ C）여 T2DM 적발생정정상관[OR（95％ CI）위1.51（1.13～2.01）]。결론지대사상관microRNA파기인결합구역다태성여 T2DM 유전역감성상관，단유대재경다적인군중득도중복험증，이급공능성연구적지지，진일보험증기병인련계。
Objective To explore the relationship between lipid metabolism related microRNA binding sites polymorphisms and the susceptibility of type 2 diabetes. Methods A case‐control study was conducted in Chinese Southeast Han population. Bioinformatics softwares were used to predict the target sites of lipid metabolism‐related microRNAs. After screening ,8 candidate SNPs in microRNA target sites were selected. By utilizing TaqMan? assay detection technology ,the genetype of SNPs in all subjects (874 T2DM patients ,896 health controls)were detected and the association between different genotypes and T2DM susceptibility was analyzed. Results After adjusting for age ,gender ,waist circumference and Bonferroni correction ,rs1065201 in CBY1 gene (T > C) had positive association with the risk of T 2DM [OR (95% CI) =1.51(1.13~2.01)]. Conclusion Polymorphisms in lipid metabolism‐related microRNA binding sites are associated with the susceptibility of T 2DM ,but the etiology requires further validation.