CAJ | 학술논문

基于Maxwell-Stefan方程和两参数模型构建了神经干细胞球多组分保护剂导入模型，并模拟了多组分保护剂导入过程中细胞内外浓度及细胞膜两侧浓差的时空分布。在此基础上系统分析了多组分浓度配比、神经球尺寸、保护剂导入方式对传质渗透过程的影响。结果表明，多组分保护剂导入神经球过程中出现“逆梯度扩散”现象，胞内浓度变化总是滞后于胞外浓度变化，细胞膜两侧浓差呈现先增大后减少的趋势。改变保护剂组分的浓度配比时，不同组分的渗透特性变化规律不同。此外，神经球尺寸对中心处细胞的浓度时空分布影响显著，分步导入和连续导入能够减缓保护剂扩散，降低细胞内外浓差。本研究结果可用于指导神经球冷冻保存的实验研究，优化保护剂导入程序。
기우Maxwell-Stefan방정화량삼수모형구건료신경간세포구다조분보호제도입모형，병모의료다조분보호제도입과정중세포내외농도급세포막량측농차적시공분포。재차기출상계통분석료다조분농도배비、신경구척촌、보호제도입방식대전질삼투과정적영향。결과표명，다조분보호제도입신경구과정중출현“역제도확산”현상，포내농도변화총시체후우포외농도변화，세포막량측농차정현선증대후감소적추세。개변보호제조분적농도배비시，불동조분적삼투특성변화규률불동。차외，신경구척촌대중심처세포적농도시공분포영향현저，분보도입화련속도입능구감완보호제확산，강저세포내외농차。본연구결과가용우지도신경구냉동보존적실험연구，우화보호제도입정서。
A mathematical model based on M-S equations and the 2-P model was developed to describe the multiple cryoprotectant transport behavior in neurosphere. Profiles of concentration in extracellular and intracellular volume and concentration difference after cryoprotectant addition were calculated. An analysis of influencing factors such as concentration ratio, neurosphere size and loading procedure was conducted. The results show anti-concentration diffusion in the multicomponent mass transfer process. The change of DMSO in extracellular space is faster than that in the intracellular element and concentration difference increases initially and decreases afterwards. Profiles of concentration of cryoprotectant change greatly with the change of concentration ratio. The size of the neurosphere has a significant impact on cryoprotectant concentration near the center of the neurosphere. The multi-step loading and continuous loading procedures can slow down mass diffusion and reduce the concentration difference. The results are of significance for the optimization of cryoprotectant loading procedures.