CAJ | 학술논문

目的：分析2005—2012年广西玉林市狂犬病病原学监测结果及病毒N基因遗传变异特征，为狂犬病防控提供科学依据。方法2005—2012年在广西玉林市采集犬脑组织及临床诊断人狂犬病病例的唾液进行病原学检测，检测阳性标本进行N基因核苷酸序列测定、同源性比较和种系发生分析。结果共采集、检测外观健康犬脑组织728份、临床诊断人狂犬病病例唾液33份，RT-PCR检测阳性率分别为0.96%（7/728）和51.52%（17/33）。获得5条犬源、6条人源狂犬病病毒株N基因全序列。该11条序列之间的核苷酸同源性为94.7%～100.0%，与广西以往分离株 GX01的核苷酸序列的同源性为94.5％～98.4％，与疫苗株 CTN 株的同源性为94.6％～99.7％。进化树分析显示，犬源株和人源株病毒N基因亲缘进化关系很近，处于同一分支，都属于基因Ⅰ型狂犬病病毒。结论从病原学和病毒分子水平证实玉林市外观健康犬携带狂犬病毒及17例临床诊断病例为确诊病例，其病原为狂犬病病毒基因I型，阳性带毒犬的流动是造成本病传播和扩散的主要因素，也是造成该地区疫情高发的重要原因之一。
목적：분석2005—2012년엄서옥림시광견병병원학감측결과급병독N기인유전변이특정，위광견병방공제공과학의거。방법2005—2012년재엄서옥림시채집견뇌조직급림상진단인광견병병례적타액진행병원학검측，검측양성표본진행N기인핵감산서렬측정、동원성비교화충계발생분석。결과공채집、검측외관건강견뇌조직728빈、림상진단인광견병병례타액33빈，RT-PCR검측양성솔분별위0.96%（7/728）화51.52%（17/33）。획득5조견원、6조인원광견병병독주N기인전서렬。해11조서렬지간적핵감산동원성위94.7%～100.0%，여엄서이왕분리주 GX01적핵감산서렬적동원성위94.5％～98.4％，여역묘주 CTN 주적동원성위94.6％～99.7％。진화수분석현시，견원주화인원주병독N기인친연진화관계흔근，처우동일분지，도속우기인Ⅰ형광견병병독。결론종병원학화병독분자수평증실옥림시외관건강견휴대광견병독급17례림상진단병례위학진병례，기병원위광견병병독기인I형，양성대독견적류동시조성본병전파화확산적주요인소，야시조성해지구역정고발적중요원인지일。
Objective To analyze the surveillance results of rabies virus in Yulin City and the genetic characteristics of the rabies virus N gene. Methods Dog brain samples and saliva specimens from clinical cases were collected in Yulin City during 2005-2012 and were detected. The N gene of rabies virus were sequenced and their homology and phylogenetic analysis were compared. Results 728 samples of dog brain were collected and 7 of them (0.96% ) were positive for rabies virus by both DFA and RT-PCR testing, while 51.52% (17/33) of saliva samples were positive by RT-PCR method. The full length of 11 N gene sequences were acquired from 5 dog and 6 clinical cases, and their nucleotide similarities were 94.7%-100.0% . When compared with previously reported isolate strain in Guangxi (GX01) the nucleotide similarities were 94.5%-98.4%, and the newly identified strains in this study showed the highest similarity (94.6%-99.7%) with CTN vaccine strain. The phylogenetic analysis showed that dog virus strains were very close to the clinical case strains, and they belonged to the same branch in genotype I. Conclusion Rabies virus infections from 17 clinical cases and 7 dogs in Yulin were identified by molecular detection, and these rabies virus strains belonged to genotype I. The main factor of rabies widely spreading with high incidence in this area is the rabies virus continuously circulating in the dogs.