CAJ | 학술논문

为研究复合菌系高效协同分解纤维素的机制，从天然富集且高效稳定分解纤维素的复合菌系WSC-9中分离获得的8株细菌纯培养进行组合，构建由3株厌氧菌（W-A、W-B、W-C）和2株好氧菌（W-3、W-5）组成且具有较高纤维素分解能力的组合，PCS培养基中50℃静止培养10 d可分解97.6%的滤纸。组合减少试验显示，W-B为纤维素分解的关键菌株，减少组合中任一菌株均会降低滤纸分解率，培养基中代谢产物乙醇、乙酸、丙酸等含量也发生变化。W-3和W-5对厌氧菌分解滤纸具有辅助作用，W-B和W-C分别对滤纸分解初期的乙醇和后期的己酸含量有重要影响，厌氧菌对培养基pH均有调节作用。
위연구복합균계고효협동분해섬유소적궤제，종천연부집차고효은정분해섬유소적복합균계WSC-9중분리획득적8주세균순배양진행조합，구건유3주염양균（W-A、W-B、W-C）화2주호양균（W-3、W-5）조성차구유교고섬유소분해능력적조합，PCS배양기중50℃정지배양10 d가분해97.6%적려지。조합감소시험현시，W-B위섬유소분해적관건균주，감소조합중임일균주균회강저려지분해솔，배양기중대사산물을순、을산、병산등함량야발생변화。W-3화W-5대염양균분해려지구유보조작용，W-B화W-C분별대려지분해초기적을순화후기적기산함량유중요영향，염양균대배양기pH균유조절작용。
To study the mechanism of cellulose degradation by composite of microbial community,the artificial microbial system with capable of cellulose degradation was constructed. The community consisted with three strains of anaerobic bacteria (W-A,W-B and W-C)and two strains of aerobic bacteria (W-3 and W-5)from eight pure culture strains that isolated from microbial community of WSC-9. The results showed that 97.6%of filter paper was decomposed after 10 days culture in PCS medium by the microbial system under static condition of 50 ℃. The reduce test showed that W-B was the key strain for cellulose degradation,the degradation rates of filter paper would be decreased when any strain was reduced,and the products of the microbial system such as ethanol,acetic,and propionic acid were also changed. The aerobic isolates of W-3 and W-5 were the important and necessary for filter paper degradation performed by anaerobic isolates. W-B and W-C also had the important effect on the content of ethanol in initial stage and caproic acid in later stage of filter paper degradation. The anaerobic isolates also had the certain regulatory effect on the culture environment pH.