哈尔滨商业大学学报(自然科学版)
哈爾濱商業大學學報(自然科學版)
합이빈상업대학학보(자연과학판)
JOURNAL OF HARBIN UNIVERSITY OF COMMERCE(NATURAL SCIENCES EDITION)
2015年
1期
28-32
,共5页
假酸浆多糖%提取%纯化
假痠漿多糖%提取%純化
가산장다당%제취%순화
Nicandra polysaccharide%extraction%purification
以假酸浆籽为原料,采用水提法、超声波提取法、微波提取法提取假酸浆多糖,经对各提取过程比较分析可知水提法为最优提取方法,提取条件为料液比1∶55、温度80℃、时间3 h,提取率为6.18%.将水提法得到的假酸浆多糖浓缩液通过醇沉、脱蛋白、脱色等工艺进行纯化,确定了最佳的提取纯化工艺.在最佳的条件下,醇沉后多糖的量可达80.5%,蛋白质脱除率可达70.8%,脱色率可达91.0%.
以假痠漿籽為原料,採用水提法、超聲波提取法、微波提取法提取假痠漿多糖,經對各提取過程比較分析可知水提法為最優提取方法,提取條件為料液比1∶55、溫度80℃、時間3 h,提取率為6.18%.將水提法得到的假痠漿多糖濃縮液通過醇沉、脫蛋白、脫色等工藝進行純化,確定瞭最佳的提取純化工藝.在最佳的條件下,醇沉後多糖的量可達80.5%,蛋白質脫除率可達70.8%,脫色率可達91.0%.
이가산장자위원료,채용수제법、초성파제취법、미파제취법제취가산장다당,경대각제취과정비교분석가지수제법위최우제취방법,제취조건위료액비1∶55、온도80℃、시간3 h,제취솔위6.18%.장수제법득도적가산장다당농축액통과순침、탈단백、탈색등공예진행순화,학정료최가적제취순화공예.재최가적조건하,순침후다당적량가체80.5%,단백질탈제솔가체70.8%,탈색솔가체91.0%.
Nicandra polysaccharide was extracted from the seed of Nicandra physaloides ( L.) Gaertn by the method of water extraction, ultrasonic extraction, microwave extraction, respectively.Through comparative analysis on the extraction process, water extraction achie-ving 6.18%extraction rate was determined to be the optimal method, in which the ratio of material to liquid was 1∶55 , the extraction temperature was 80 ℃, and the extraction time was 3 h.The treatments of ethanol precipitation, deproteinization and decolorization were carried out for purifying to form the final polysaccharide.After the ethanol precipitation, the content of polysaccharide reached 80.5%, the protein removal rate reached 70.8%and the decolorization rate reached 91.0%.