CAJ | 학술논문

目的：探讨链脲佐菌素（Streptozotocin ，STZ）诱导的糖尿病大鼠坐骨神经传导速度（NCV）变化，初步确立糖尿病周围神经病（diabetic peripheral neuropathy ，DPN）大鼠模型的构建和评价方法。方法：选用SD大鼠，随机分为正常对照组和模型组，模型组腹腔注射STZ。检测动物体质量、血糖、触觉、神经传导速度（NCV）变化，并取大鼠坐骨神经组织进行 HE染色，观察其形态结构变化。结果：与正常对照组比较，给予STZ后，模型组动物体质量逐渐下降，4 w 后比较差异有显著意义（ P＜0．05），血糖显著升高（P＜0．001）；50％机械缩足阈值，于注射STZ 2 w 后明显下降（P＜0．05），注射STZ后4 w明显上升（P＜0．05）；给予STZ后4 w ，模型组大鼠运动神经传导速度（MCV）和感觉神经传导速度（SCV）显著减慢（P＜0．05），坐骨神经组织神经纤维松散，密度不均匀。结论：注射STZ 4 W后，大鼠MCV和SCV减慢，触觉敏感度呈双相变化，坐骨神经出现病理形态结构，成功复制出DPN大鼠模型。
목적：탐토련뇨좌균소（Streptozotocin ，STZ）유도적당뇨병대서좌골신경전도속도（NCV）변화，초보학립당뇨병주위신경병（diabetic peripheral neuropathy ，DPN）대서모형적구건화평개방법。방법：선용SD대서，수궤분위정상대조조화모형조，모형조복강주사STZ。검측동물체질량、혈당、촉각、신경전도속도（NCV）변화，병취대서좌골신경조직진행 HE염색，관찰기형태결구변화。결과：여정상대조조비교，급여STZ후，모형조동물체질량축점하강，4 w 후비교차이유현저의의（ P＜0．05），혈당현저승고（P＜0．001）；50％궤계축족역치，우주사STZ 2 w 후명현하강（P＜0．05），주사STZ후4 w명현상승（P＜0．05）；급여STZ후4 w ，모형조대서운동신경전도속도（MCV）화감각신경전도속도（SCV）현저감만（P＜0．05），좌골신경조직신경섬유송산，밀도불균균。결론：주사STZ 4 W후，대서MCV화SCV감만，촉각민감도정쌍상변화，좌골신경출현병리형태결구，성공복제출DPN대서모형。
Objective:To investigate the changes siatic nerve conduction velocity in diabetic rats in‐duced by streptozotocin so as to establish and evaluate experimental diabetic peripheral neuropathy model rats .Methods:SD rats were randomly divided into 2 groups:the control group ,and the model group .Rats in the model group were injected intraperitoneally with STZ .Body weight ,fasting blood glucose and 50%of mechanical withdraw threshold were measured on time .Nerve conduction velocity was tested at the end of the experiment .Sciatic nerve was stained with H&E to observe the pathological changes .Results:Com‐pared with the control group ,rats in the DPN group showed significant decrease in weight (P<0 .05)3 weeks after injection of STZ and increase in fasting blood glucose (P<0 .05) 3 days after injecting STZ 50% mechanical withdraw threshold decreased significantly 2 weeks after injection of STZ (P< 0 .05) and decreased significantly 4 weeks after injection of STZ(P < 0 .05) .Motor nerve conduction velocity and sensory nerve conduction velocity declined significantly (P<0 .05) .Fibers of sciatic nerves arranged loosely ,in which the density was uneven .Conclusion:The experimental diabetic peripheral neuropathy model can be established by injecting with STZ ,tactile or pain sensitivity increases 2 weeks later and then decreases 4 weeks later ,and nerve conduction velocity and density of fibers of sciatic nerves decrease later 4 w eeks .