CAJ | 학술논문

[目的]该研究旨在探索番茄 NAC转录因子与抗低温的关系。[方法]以番茄幼苗为试材，采用 RT-PCR方法克隆 NAC1基因 N端保守域，并利用 RT-qPCR技术检测该基因在4℃低温 l处理下的表达情况。[结果]在番茄根、茎、叶中 NAC1基因均能表达，表达量随低温处理时间的变化而发生相应的变化。在番茄的不同器官中 NAC1表达水平也有所不同，根中的表达水平整体高于茎和叶片中。[结论]该研究为进一步研究番茄 NAC1基因的生物学功能奠定理论基础。
[목적]해연구지재탐색번가 NAC전록인자여항저온적관계。[방법]이번가유묘위시재，채용 RT-PCR방법극륭 NAC1기인 N단보수역，병이용 RT-qPCR기술검측해기인재4℃저온 l처리하적표체정황。[결과]재번가근、경、협중 NAC1기인균능표체，표체량수저온처리시간적변화이발생상응적변화。재번가적불동기관중 NAC1표체수평야유소불동，근중적표체수평정체고우경화협편중。[결론]해연구위진일보연구번가 NAC1기인적생물학공능전정이론기출。
Objective] This study aimed to explore the relationship between NAC tran-scription factor and resistance to low temperature in tomato. [Method] Tomato seedlings were used as experimental materials to clone the N-terminal conserved domain of NAC1 gene with RT-PCR and investigate the expression patterns of NAC1 gene under 4 ℃ with real-time quantitative PCR (RT-qPCR). [Result] NAC1 gene was expressed in roots, stems and leaves of tomato, but the expression level varied with the change of treatment duration. Moreover, the expression levels of NAC1 varied among different organs of tomato. The expression level of NAC1 in roots was much higher than that in stems and leaves. [Conclusion] This study laid a theoretical foundation for further investigation of the biological function of NAC1 gene in tomato.