CAJ | 학술논문

伊莎褐88日龄雏鸡120只，随机分为6组，3个对照组（常温组、高温组、高温 Vc组）和3个中草药高温组（高剂量组、中剂量组、低剂量组）。常温组在14~25℃环境下饲养，其余5组在28~39℃环境下饲养.试验全程共10天，在试验的第1、4、8、10天每组各剖杀5只并取其十二指肠。经常规方法制成组织切片后 H.E染色，测定各十二指肠的黏膜厚度、绒毛长度、隐窝深度。结果显示：热应激状态下，十二指肠黏膜厚度和绒毛长度均显著低于常温组，且绒毛稀疏。但是高温条件下，中草药高剂量组、中剂量组各项指标均高于高温组和高温 Vc组，特别是在试验的第8天和第10天差异极显著，并且中草药高剂量组各指标显著高于中草药中低剂量组。该处方对热应激鸡十二指肠粘膜的保护作用强于高温 Vc组。在热应激条件下中草药有效减弱了十二指肠粘膜的组织损伤。
이사갈88일령추계120지，수궤분위6조，3개대조조（상온조、고온조、고온 Vc조）화3개중초약고온조（고제량조、중제량조、저제량조）。상온조재14~25℃배경하사양，기여5조재28~39℃배경하사양.시험전정공10천，재시험적제1、4、8、10천매조각부살5지병취기십이지장。경상규방법제성조직절편후 H.E염색，측정각십이지장적점막후도、융모장도、은와심도。결과현시：열응격상태하，십이지장점막후도화융모장도균현저저우상온조，차융모희소。단시고온조건하，중초약고제량조、중제량조각항지표균고우고온조화고온 Vc조，특별시재시험적제8천화제10천차이겁현저，병차중초약고제량조각지표현저고우중초약중저제량조。해처방대열응격계십이지장점막적보호작용강우고온 Vc조。재열응격조건하중초약유효감약료십이지장점막적조직손상。
Objective] This study aimed to investigate the effects of traditional Chi-nese herbal medicine on the structure of duodenal mucosa of chickens under heat stress. [Method] One hundred and twenty 88-day-old lsa Brown chickens were ran-domly divided into six groups, including three control groups (normal temperature control group, high temperature control group, high temperature Vc control group) and three high-temperature administration groups (high-dose administration group, moderate-dose administration group, low-dose administration group). Chickens in nor-mal temperature control group were reared at 14-25 ℃, and those in other five groups were reared at 28-39 ℃. The experiment lasted 10 d. Five chickens in each group were euthanized at 1, 4, 8 and 10 d post-treatment, respectively. The duodenal mucosa was col ected and prepared into tissue slices with the conven-tional method for hematoxylin-eosin (HE) staining. Mucosal thickness, vil us length and crypt depth of duodenal mucosa were measured. [Result] Under heat stress, duodenal mucosal thickness and vil us length were both significantly lower than those in normal temperature control group, and the duodenal vil i were scarce and thin. However, under high temperature conditions, various indicators in high-dose and moderate-dose administration groups were higher than those in high tempera-ture control group and high temperature Vc control group, which exhibited extremely significant differences at 8 and 10 d post-treatment, especial y. Moreover, various in-dicators in high-dose administration group were significantly higher than those in moderate-dose and low-dose administration groups. The traditional Chinese herbal medicine prescription exhibited higher protective effects on duodenal mucosa of chickens under heat stress compared with high temperature Vc control group. [Con-clusion] Under heat stress, traditional Chinese herbal medicine reduced effectively the duodenal mucosa damage in chickens.