农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2015年
1期
93-98
,共6页
王宝祥%谭维娜%卢百关%宋兆强%徐大勇
王寶祥%譚維娜%盧百關%宋兆彊%徐大勇
왕보상%담유나%로백관%송조강%서대용
水稻%‘连粳7号’%黑条矮缩病%抗性分析
水稻%‘連粳7號’%黑條矮縮病%抗性分析
수도%‘련갱7호’%흑조왜축병%항성분석
Rice (Oryza sativa L.)%Lianjing 7%RBSDD%Resistance analysis
为分析水稻品种‘连粳7号’对水稻黑条矮缩病的抗性，获得与抗性基因紧密连锁的分子标记，通过田间自然接种和室内人工接种方法，对‘连粳7号’及其与感病品种‘培矮64’衍生来的 F2：3群体进行黑条矮缩病抗性鉴定。结果表明，‘连粳7号’发病率最高为12.25%，最低为5.5%。‘连粳7号’的亲本中，仅‘中粳川-2’的抗性与‘连粳7号’相近。另外，‘连粳7号’对灰飞虱不具有抗性，所以‘连粳7号’对黑条矮缩病的抗性是品种本身对病毒的抗性所致。连粳7号/培矮64F2：3群体中，各个家系表型呈现连续分布，并具有超亲分离特点，说明抗性由多基因控制。最终，通过 BSA法获得与抗性位点紧密连锁的标记RM287。综上，‘连粳7号’对黑条矮缩病具有较强抗性，抗性来自亲本‘中粳川-2’，标记RM287将为利用分子标记辅助培育抗黑条矮缩病水稻新品种提供有力工具。
為分析水稻品種‘連粳7號’對水稻黑條矮縮病的抗性，穫得與抗性基因緊密連鎖的分子標記，通過田間自然接種和室內人工接種方法，對‘連粳7號’及其與感病品種‘培矮64’衍生來的 F2：3群體進行黑條矮縮病抗性鑒定。結果錶明，‘連粳7號’髮病率最高為12.25%，最低為5.5%。‘連粳7號’的親本中，僅‘中粳川-2’的抗性與‘連粳7號’相近。另外，‘連粳7號’對灰飛虱不具有抗性，所以‘連粳7號’對黑條矮縮病的抗性是品種本身對病毒的抗性所緻。連粳7號/培矮64F2：3群體中，各箇傢繫錶型呈現連續分佈，併具有超親分離特點，說明抗性由多基因控製。最終，通過 BSA法穫得與抗性位點緊密連鎖的標記RM287。綜上，‘連粳7號’對黑條矮縮病具有較彊抗性，抗性來自親本‘中粳川-2’，標記RM287將為利用分子標記輔助培育抗黑條矮縮病水稻新品種提供有力工具。
위분석수도품충‘련갱7호’대수도흑조왜축병적항성，획득여항성기인긴밀련쇄적분자표기，통과전간자연접충화실내인공접충방법，대‘련갱7호’급기여감병품충‘배왜64’연생래적 F2：3군체진행흑조왜축병항성감정。결과표명，‘련갱7호’발병솔최고위12.25%，최저위5.5%。‘련갱7호’적친본중，부‘중갱천-2’적항성여‘련갱7호’상근。령외，‘련갱7호’대회비슬불구유항성，소이‘련갱7호’대흑조왜축병적항성시품충본신대병독적항성소치。련갱7호/배왜64F2：3군체중，각개가계표형정현련속분포，병구유초친분리특점，설명항성유다기인공제。최종，통과 BSA법획득여항성위점긴밀련쇄적표기RM287。종상，‘련갱7호’대흑조왜축병구유교강항성，항성래자친본‘중갱천-2’，표기RM287장위이용분자표기보조배육항흑조왜축병수도신품충제공유력공구。
To analyze the resistance of Lianjing 7 against rice black-streaked dwarf disease and find the molecular markers closely linked with the resistance gene, an F2:3 population derived from an RBSDD-resistant cultivar Lianjing 7 and an RBS-DD-susceptible cultivar Peiai 64, was evaluated using natural infection methods and artificial inoculation by scoring the disease severity rating. The results showed Lian-jing 7 was resistant to RBSDD, with the incidence between 5.5% and 12.25% in al tests. For the parents of Lianjing 7, only Zhongjingchuan-2 was resistant to RBSDD. Besides this, Lianjing 7 assumed an antixenosis and antibiosis score similar with susceptible variety Wuyujing 3, which indicates that so RBSDD-resistance of Lianjing 7 is mainly caused by the resistance specifical y to RBSDV. ln the F2:3 population of Lianjing 7/Peiai 64, the disease severity rates showed a continuous distribution with transgressive segregation, it indicated the resistance of Lianjing 7 was con-trol ed by multi-genes. The marker RM287 was determined closely linked with the resistance locus via BSA method. To conclude, Lianjing 7 has a high resistance to RBSDD, which is conferred with by Zhongjingchuan-2, and the marker RM287 should be useful for breeding novel RBSDD-resistant cultivars via marker-assisted selection (MAS).
