实验与检验医学
實驗與檢驗醫學
실험여검험의학
EXPERIMENTAL AND LABORATORY MEDICINE
2015年
1期
31-33
,共3页
黄劲柏%吴志群%苏明珍%李北坤
黃勁柏%吳誌群%囌明珍%李北坤
황경백%오지군%소명진%리북곤
全自动毛细管电泳仪%HbE病%检测%价值
全自動毛細管電泳儀%HbE病%檢測%價值
전자동모세관전영의%HbE병%검측%개치
Automatic capillary electrophoresis apparatus%HbE Disease%Detection%Value
目的：探讨全自动毛细管电泳仪在HbE病检测中的应用价值，为今后临床检测提供参考和借鉴。方法选取2013年10月至2014年10月期间在我院接受检测的孕妇及其配偶7311例的样本为研究对象，根据其临床资料进行回顾性分析和研究，探讨全自动毛细管电泳仪在HbE病检测中的应用价值。结果接受检测的7311例样本中共检测出HbE带25例，其检出率为0.34%。 HbE带定量最小值为14.2%，最大值为84.6%，平均值为(29.68±14.56)%，其中包括HbE复合α地贫5例，HbE复合β地贫为3例，HbE杂合子15例，HbE纯合子2例；地贫基因检测结果与全自动毛细管电泳仪的检查结果相比没有差异，P＞0.05，符合率为100%。结论全自动毛细管电泳仪在HbE病检测中发挥着重要的作用，不仅可以分析出HbE带，还可以区分出HbE病是杂合子或是纯合子，是筛查血红蛋白HbE的理想方式。
目的：探討全自動毛細管電泳儀在HbE病檢測中的應用價值，為今後臨床檢測提供參攷和藉鑒。方法選取2013年10月至2014年10月期間在我院接受檢測的孕婦及其配偶7311例的樣本為研究對象，根據其臨床資料進行迴顧性分析和研究，探討全自動毛細管電泳儀在HbE病檢測中的應用價值。結果接受檢測的7311例樣本中共檢測齣HbE帶25例，其檢齣率為0.34%。 HbE帶定量最小值為14.2%，最大值為84.6%，平均值為(29.68±14.56)%，其中包括HbE複閤α地貧5例，HbE複閤β地貧為3例，HbE雜閤子15例，HbE純閤子2例；地貧基因檢測結果與全自動毛細管電泳儀的檢查結果相比沒有差異，P＞0.05，符閤率為100%。結論全自動毛細管電泳儀在HbE病檢測中髮揮著重要的作用，不僅可以分析齣HbE帶，還可以區分齣HbE病是雜閤子或是純閤子，是篩查血紅蛋白HbE的理想方式。
목적：탐토전자동모세관전영의재HbE병검측중적응용개치，위금후림상검측제공삼고화차감。방법선취2013년10월지2014년10월기간재아원접수검측적잉부급기배우7311례적양본위연구대상，근거기림상자료진행회고성분석화연구，탐토전자동모세관전영의재HbE병검측중적응용개치。결과접수검측적7311례양본중공검측출HbE대25례，기검출솔위0.34%。 HbE대정량최소치위14.2%，최대치위84.6%，평균치위(29.68±14.56)%，기중포괄HbE복합α지빈5례，HbE복합β지빈위3례，HbE잡합자15례，HbE순합자2례；지빈기인검측결과여전자동모세관전영의적검사결과상비몰유차이，P＞0.05，부합솔위100%。결론전자동모세관전영의재HbE병검측중발휘착중요적작용，불부가이분석출HbE대，환가이구분출HbE병시잡합자혹시순합자，시사사혈홍단백HbE적이상방식。
Objective To explore application value of full-automatic capillary electrophoresis apparatus in HbE disease de-tection, and provide reference for clinical detection in the future. Methods A total of 7311 specimens of pregnant women and their spouses who received detections during the period from October, 2013 to October, 2014 were selected as research subjects, retrospective analysis and research were conducted according to their clinical data, and the application value of automatic capillary electrophoresis apparatus in HbE disease detection was explored. Results HbE bands were detected in 25 of 7311 specimens, and the detectable rate was 0.34%. The minimum and maximum quantitative value of HbE band was 14.2% and 84.6% respectively, the average value was (29.68 ±14.56)%, including 5 cases of HbE compound α thalassemia, 3 cases of HbE compound β tha-lassemia, 15 cases of HbE heterozygote and 2 cases of homozygous HbE, and there was no difference between examination results of thalassemia gene detection and that of fully automatic capillary electrophoresis apparatus(P>0.05), and the coincidence rate was 100%. Conclusions Fully automatic capillary electrophoresis apparatus plays an important role in the detection of HbE disease, it is not only capable of analyzing HbE band, but also can distinguish if HbE disease is heterozygous or homozygous. It is an ideal way to screening HbE hemoglobin.
